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Int. J. Mol. Sci. 2012, 13(8), 10176-10192; doi:10.3390/ijms130810176
Article

Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation

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Received: 5 June 2012; in revised form: 3 August 2012 / Accepted: 7 August 2012 / Published: 15 August 2012
(This article belongs to the Special Issue Advances in Molecular Oncology)
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Abstract: Helicobacter pylori (H. pylori), the human stomach pathogen, lives on the inner surface of the stomach and causes chronic gastritis, peptic ulcer, and gastric cancer. Plasma membrane repair response is a matter of life and death for human cells against physical and biological damage. We here test the hypothesis that H. pylori also causes plasma membrane disruption injury, and that not only a membrane repair response but also a cell proliferation response are thereby activated. Vacuolating cytotoxin A (VacA) and cytotoxin-associated gene A (CagA) have been considered to be major H. pylori virulence factors. Gastric cancer cells were infected with H. pylori wild type (vacA+/cagA+), single mutant (ΔvacA or ΔcagA) or double mutant (ΔvacA/ΔcagA) strains and plasma membrane disruption events and consequent activation of membrane repair components monitored. H. pylori disrupts the host cell plasma membrane, allowing localized dye and extracellular Ca2+ influx. Ca2+-triggered members of the annexin family, A1 and A4, translocate, in response to injury, to the plasma membrane, and cell surface expression of an exocytotic maker of repair, LAMP-2, increases. Additional forms of plasma membrane disruption, unrelated to H. pylori exposure, also promote host cell proliferation. We propose that H. pylori activation of a plasma membrane repair is pro-proliferative. This study might therefore provide new insight into potential mechanisms of H. pylori-induced gastric carcinogenesis.
Keywords: plasma membrane repair; Helicobacter pylori; cell proliferation; annexin A4 plasma membrane repair; Helicobacter pylori; cell proliferation; annexin A4
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lin, L.-L.; Huang, H.-C.; Ogihara, S.; Wang, J.-T.; Wu, M.-C.; McNeil, P.L.; Chen, C.-N.; Juan, H.-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. Int. J. Mol. Sci. 2012, 13, 10176-10192.

AMA Style

Lin L-L, Huang H-C, Ogihara S, Wang J-T, Wu M-C, McNeil PL, Chen C-N, Juan H-F. Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation. International Journal of Molecular Sciences. 2012; 13(8):10176-10192.

Chicago/Turabian Style

Lin, Li-Ling; Huang, Hsuan-Cheng; Ogihara, Satoshi; Wang, Jin-Town; Wu, Meng-Chuan; McNeil, Paul L.; Chen, Chiung-Nien; Juan, Hsueh-Fen. 2012. "Helicobacter pylori Disrupts Host Cell Membranes, Initiating a Repair Response and Cell Proliferation." Int. J. Mol. Sci. 13, no. 8: 10176-10192.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert