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Structural and Oxidative Changes in the Kidney of Crucian Carp Induced by Silicon-Based Quantum Dots
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania
Department of Preclinical Sciences, University of Agricultural Sciences and Veterinary Medicine, 105 Splaiul Independentei, Bucharest 050097, Romania
Laser Department, National Institute of Laser, Plasma and Radiation Physics, 409 Atomistilor, Bucharest-Magurele 077125, Romania
Department of Mathematics, University of Agricultural Sciences and Veterinary Medicine, 59 Marasti Bd., Bucharest 011464, Romania
* Author to whom correspondence should be addressed.
Received: 2 July 2012; in revised form: 28 July 2012 / Accepted: 9 August 2012 / Published: 16 August 2012
Abstract: Silicon-based quantum dots were intraperitoneally injected in Carassius auratus gibelio specimens and, over one week, the effects on renal tissue were investigated by following their distribution and histological effects, as well as antioxidative system modifications. After three and seven days, detached epithelial cells from the basal lamina, dilated tubules and debris in the lumen of tubules were observed. At day 7, nephrogenesis was noticed. The reduced glutathione (GSH) concentration decreased in the first three days and started to rise later on. The superoxide dismutase (SOD) activity increased only after one week, whereas catalase (CAT) was up-regulated in a time-dependent manner. The activities of glutathione reductase (GR) and glutathione peroxidise (GPX) decreased dramatically by approximately 50% compared to control, whereas the glutathione-S-transferase (GST) and glucose-6-phosphate dehydrogenase (G6PDH) increased significantly after 3 and 7 days of treatment. Oxidative modifications of proteins and the time-dependent increase of Hsp70 expression were also registered. Our data suggest that silicon-based quantum dots induced oxidative stress followed by structural damages. However, renal tissue is capable of restoring its integrity by nephron development.
Keywords: quantum dots; fish kidney; oxidative stress
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Petrache, S.N.; Stanca, L.; Serban, A.I.; Sima, C.; Staicu, A.C.; Munteanu, M.C.; Costache, M.; Burlacu, R.; Zarnescu, O.; Dinischiotu, A. Structural and Oxidative Changes in the Kidney of Crucian Carp Induced by Silicon-Based Quantum Dots. Int. J. Mol. Sci. 2012, 13, 10193-10211.
Petrache SN, Stanca L, Serban AI, Sima C, Staicu AC, Munteanu MC, Costache M, Burlacu R, Zarnescu O, Dinischiotu A. Structural and Oxidative Changes in the Kidney of Crucian Carp Induced by Silicon-Based Quantum Dots. International Journal of Molecular Sciences. 2012; 13(8):10193-10211.
Petrache, Sorina Nicoleta; Stanca, Loredana; Serban, Andreea Iren; Sima, Cornelia; Staicu, Andreia Cristina; Munteanu, Maria Cristina; Costache, Marieta; Burlacu, Radu; Zarnescu, Otilia; Dinischiotu, Anca. 2012. "Structural and Oxidative Changes in the Kidney of Crucian Carp Induced by Silicon-Based Quantum Dots." Int. J. Mol. Sci. 13, no. 8: 10193-10211.