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MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells
Department of Surgery, The First Hospital of Shanxi Medical University, Taiyuan 030001, China
Department of Thoracic Surgery, Shanxi Cancer Hospital, Taiyuan 030001, China
Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology, Beijing 100005, China
* Author to whom correspondence should be addressed.
Received: 23 May 2012; in revised form: 19 June 2012 / Accepted: 6 July 2012 / Published: 16 July 2012
Abstract: MicroRNAs (miRNAs) are important regulators of multiple cellular processes, and the deregulation of miRNA is a common event in diverse human diseases, particularly cancer. However, the mechanisms underlying the relationship between disordered miRNA expression and tumorigenesis have remained largely unknown. In this study, we demonstrated the down-regulation of miR-125b in hepatocellular carcinoma (HCC) tissues and HCC cell lines by Northern blot and quantitative RT-PCR analyses. The ectopic expression of miR-125b reduced the cellular proliferation and cell cycle progression of HCC cells by targeting Mcl-1 and IL6R. Furthermore, the miR-125b-induced inhibition of cell proliferation was rescued by the expression of Mcl-1 or IL6R variants that lacked 3' UTRs. Thus, this study revealed the differential expression of miR-125b in HCC cells and elucidated its potential as a tumor suppressor in HCC development.
Keywords: hepatocellular carcinoma; miR-125b; Mcl-1; IL6R
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Jia, H.-Y.; Wang, Y.-X.; Yan, W.-T.; Li, H.-Y.; Tian, Y.-Z.; Wang, S.-M.; Zhao, H.-L. MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells. Int. J. Mol. Sci. 2012, 13, 8762-8774.
Jia H-Y, Wang Y-X, Yan W-T, Li H-Y, Tian Y-Z, Wang S-M, Zhao H-L. MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells. International Journal of Molecular Sciences. 2012; 13(7):8762-8774.
Jia, Hong-Yan; Wang, Yu-Xuan; Yan, Wen-Ting; Li, Hui-Yu; Tian, Yan-Zhang; Wang, Shi-Ming; Zhao, Hao-Liang. 2012. "MicroRNA-125b Functions as a Tumor Suppressor in Hepatocellular Carcinoma Cells." Int. J. Mol. Sci. 13, no. 7: 8762-8774.