Int. J. Mol. Sci. 2012, 13(5), 5607-5627; doi:10.3390/ijms13055607
Article

Modification of MCF-10A Cells with Pioglitazone and Serum-Rich Growth Medium Increases Soluble Factors in the Conditioned Medium, Likely Reducing BT-474 Cell Growth

1,2,* email, 1email, 1email, 1,email and 2email
Received: 13 February 2012; in revised form: 25 April 2012 / Accepted: 28 April 2012 / Published: 10 May 2012
(This article belongs to the Special Issue Advances in Molecular Immunology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In the present study, we aimed to preincubate MCF-10A cells with pioglitazone and/or serum-rich growth media and to determine adhesive and non-adhesive interactions of the preincubated MCF-10A cells with BT-474 cells. For this purpose, the MCF-10A cells were preincubated with pioglitazone and/or serum-rich growth media, at appropriate concentrations, for 1 week. The MCF-10A cells preincubated with pioglitazone and/or serum-rich growth media were then co-cultured adhesively and non-adhesively with BT-474 cells for another week. Co-culture of BT-474 cells with the preincubated MCF-10A cells, both adhesively and non-adhesively, reduced the growth of the cancer cells. The inhibitory effect of the preincubated MCF-10A cells against the growth of BT-474 cells was likely produced by increasing levels of soluble factors secreted by the preincubated MCF-10A cells into the conditioned medium, as immunoassayed by ELISA. However, only an elevated level of a soluble factor distinguished the conditioned medium collected from the MCF-10A cells preincubated with pioglitazone and serum-rich growth medium than that with pioglitazone alone. This finding was further confirmed by the induction of the soluble factor transcript expression in the preincubated MCF-10A cells, as determined using real-time PCR, for the above phenomenon. Furthermore, modification of the MCF-10A cells through preincubation did not change the morphology of the cells, indicating that the preincubated cells may potentially be injected into mammary fat pads to reduce cancer growth in patients or to be used for others cell-mediated therapy.
Keywords: adhesive and non-adhesive cell interaction; cell growth; human breast cancer; pioglitazone; serum-rich growth medium; tumourigenic and non-tumourigenic cells
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MDPI and ACS Style

Khoo, B.Y.; Miswan, N.; Balaram, P.; Nadarajan, K.; Elstner, E. Modification of MCF-10A Cells with Pioglitazone and Serum-Rich Growth Medium Increases Soluble Factors in the Conditioned Medium, Likely Reducing BT-474 Cell Growth. Int. J. Mol. Sci. 2012, 13, 5607-5627.

AMA Style

Khoo BY, Miswan N, Balaram P, Nadarajan K, Elstner E. Modification of MCF-10A Cells with Pioglitazone and Serum-Rich Growth Medium Increases Soluble Factors in the Conditioned Medium, Likely Reducing BT-474 Cell Growth. International Journal of Molecular Sciences. 2012; 13(5):5607-5627.

Chicago/Turabian Style

Khoo, Boon Yin; Miswan, Noorizan; Balaram, Prabha; Nadarajan, Kalpanah; Elstner, Elena. 2012. "Modification of MCF-10A Cells with Pioglitazone and Serum-Rich Growth Medium Increases Soluble Factors in the Conditioned Medium, Likely Reducing BT-474 Cell Growth." Int. J. Mol. Sci. 13, no. 5: 5607-5627.

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