Int. J. Mol. Sci. 2012, 13(5), 5628-5644; doi:10.3390/ijms13055628
Article

Anti-TNF-α Activity of Portulaca oleracea in Vascular Endothelial Cells

1,†email, 2,†email, 1,3email, 1,3,* email and 1,3,* email
Received: 20 February 2012; in revised form: 20 April 2012 / Accepted: 2 May 2012 / Published: 10 May 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Vascular inflammation plays a key role in the pathogenesis and progression of atherosclerosis, a main complication of diabetes. The present study investigated whether an aqueous extract of Portulaca oleracea (AP) prevents the TNF-α-induced vascular inflammatory process in the human umbilical vein endothelial cell (HUVEC). The stimulation of TNF-α induced overexpression of adhesion molecules affects vascular cell adhesion molecule (VCAM)-1, intercellular adhesion molecule (ICAM)-1 and E-selectin for example. However, AP significantly suppressed TNF-α-induced over-expression of these adhesion molecules in a dose-dependent manner. In addition, pretreatment with AP dose-dependently reduced an increase of the adhesion of HL-60 cells to TNF-α-induced HUVEC. Furthermore, we observed that stimulation of TNF-α significantly increased intracellular reactive oxygen species (ROS) production. However, pretreatment with AP markedly blocked TNF-α-induced ROS production in a dose-dependent manner. The western blot and immunofluorescence analysis showed that AP inhibited the translocation of p65 NF-κB to the nucleus. In addition, AP suppressed the TNF-α-induced degradation of IκB-α and attenuated the TNF-α-induced NF-κB binding. AP also effectively reduced TNF-α-induced mRNA expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-8 in a dose-dependent manner. Taken together, AP prevents the vascular inflammatory process through the inhibition of intracellular ROS production and NF-κB activation as well as the reduction of adhesion molecule expression in TNF-α-induced HUVEC. These results suggested that AP might have a potential therapeutic effect by inhibiting the vascular inflammation process in vascular diseases such as atherosclerosis.
Keywords: Portulaca oleracea; inflammation; NF-κB; reactive oxygen species (ROS); atherosclerosis
PDF Full-text Download PDF Full-Text [455 KB, uploaded 19 June 2014 04:16 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Lee, A.S.; Kim, J.S.; Lee, Y.J.; Kang, D.G.; Lee, H.S. Anti-TNF-α Activity of Portulaca oleracea in Vascular Endothelial Cells. Int. J. Mol. Sci. 2012, 13, 5628-5644.

AMA Style

Lee AS, Kim JS, Lee YJ, Kang DG, Lee HS. Anti-TNF-α Activity of Portulaca oleracea in Vascular Endothelial Cells. International Journal of Molecular Sciences. 2012; 13(5):5628-5644.

Chicago/Turabian Style

Lee, An Sook; Kim, Jin Sook; Lee, Yun Jung; Kang, Dae Gill; Lee, Ho Sub. 2012. "Anti-TNF-α Activity of Portulaca oleracea in Vascular Endothelial Cells." Int. J. Mol. Sci. 13, no. 5: 5628-5644.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert