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• Cheng, H
• Lee, Y
• Hsiung, D
• Tseng, M
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• Cheng, H
• Lee, Y
• Hsiung, D
• Tseng, M
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• Cheng, H
• Lee, Y
• Hsiung, D
• Tseng, M

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Int. J. Mol. Sci. 2012, 13(5), 5598-5606; doi:10.3390/ijms13055598

Article

Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa

Hui-Ling Cheng ¹ , Yan-Horn Lee ² , Dai-Shion Hsiung ³  and Mei-Chen Tseng ^3,*

¹ Department of Environmental Resources Management, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan ² Biotechnology Division, Fisheries Research Institute Council of Agriculture, Tungkang, Pingtung 928, Taiwan ³ Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan

* Author to whom correspondence should be addressed.

Received: 20 February 2012; in revised form: 22 April 2012 / Accepted: 24 April 2012 / Published: 9 May 2012

(This article belongs to the Section Molecular Diagnostics)

Download PDF Full-Text [229 KB, uploaded 9 May 2012 14:15 CEST]

Abstract: The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. formosa population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the genetic diversity of P. formosa. With the exception of the Pfo15 locus, all of the remaining loci were polymorphic with allelic numbers ranging from 3–14. Heterozygosity within all 17 polymorphic loci ranged from 0.2–0.95 with an average of 0.55, which suggested that these loci are proper markers for studying population genetics. After we tested cross-specific amplification, eight and six primer sets could be successfully used for the amplification of microsatellite loci in morphologically similar Eriocheir sinensis and E. japonica, respectively; this suggests that they are useful markers for closely related species.

Keywords: catadromous; crab; cross-specific amplification; polymorphic loci

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