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Int. J. Mol. Sci. 2012, 13(4), 4351-4366; doi:10.3390/ijms13044351
Article

Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines

1
,
2
,
3
,
3,4
 and
3,4,*
1 Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan 2 Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan 3 Department of Nutrition and Health Science, Fooyin University, Kaohsiung 83102, Taiwan 4 Research Center of Health Food, Fooyin University, Kaohsiung 83102, Taiwan
* Author to whom correspondence should be addressed.
Received: 1 February 2012 / Revised: 5 March 2012 / Accepted: 16 March 2012 / Published: 5 April 2012
(This article belongs to the Special Issue UV-Induced Cell Death)
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Abstract

Ultraviolet C (UVC) is a DNA damage inducer, and 20 J/m2 of UVC irradiation caused cell growth inhibition and induced cell death after exposure for 24–36 h. The growth of NIH 3T3 cells was significantly suppressed at 24 h after UVC irradiation whereas the proliferation of A431 cells was inhibited until 36 h after UVC irradiation. UVC irradiation increased COX-2 expression and such up-regulation reached a maximum during 3–6 h in NIH 3T3 cells. In contrast, UVC-induced COX-2 reached a maximum after 24–36 h in A431 cells. Measuring prostaglandin E2 (PGE2) level showed a biphasic profile that PGE2 release was rapidly elevated in 1–12 h after UVC irradiation and increased again at 24 h in both cell lines. Treatment with the selective COX-2 inhibitor, SC-791, during maximum expression of COX-2 induction, attenuated the UVC induced-growth inhibition in NIH 3T3 cells. In contrast, SC-791 treatment after UVC irradiation enhanced death of A431 cells. These data showed that the patterns of UVC-induced PGE2 secretion from NIH 3T3 cells and A431 cells were similar despite the differential profile in UVC-induced COX-2 up-regulation. Besides, COX-2 might play different roles in cellular response to UVC irradiation in various cell lines.
Keywords: ultraviolet C (UVC); cyclooxygenases-2 (COX-2); prostaglandin E2 (PGE2); NIH 3T3 cells; A431 cells ultraviolet C (UVC); cyclooxygenases-2 (COX-2); prostaglandin E2 (PGE2); NIH 3T3 cells; A431 cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tai, M.-H.; Weng, C.-H.; Mon, D.-P.; Hu, C.-Y.; Wu, M.-H. Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines. Int. J. Mol. Sci. 2012, 13, 4351-4366.

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