Int. J. Mol. Sci. 2012, 13(4), 4351-4366; doi:10.3390/ijms13044351
Article

Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines

1 Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan 2 Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan 3 Department of Nutrition and Health Science, Fooyin University, Kaohsiung 83102, Taiwan 4 Research Center of Health Food, Fooyin University, Kaohsiung 83102, Taiwan
* Author to whom correspondence should be addressed.
Received: 1 February 2012; in revised form: 5 March 2012 / Accepted: 16 March 2012 / Published: 5 April 2012
(This article belongs to the Special Issue UV-Induced Cell Death)
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Abstract: Ultraviolet C (UVC) is a DNA damage inducer, and 20 J/m2 of UVC irradiation caused cell growth inhibition and induced cell death after exposure for 24–36 h. The growth of NIH 3T3 cells was significantly suppressed at 24 h after UVC irradiation whereas the proliferation of A431 cells was inhibited until 36 h after UVC irradiation. UVC irradiation increased COX-2 expression and such up-regulation reached a maximum during 3–6 h in NIH 3T3 cells. In contrast, UVC-induced COX-2 reached a maximum after 24–36 h in A431 cells. Measuring prostaglandin E2 (PGE2) level showed a biphasic profile that PGE2 release was rapidly elevated in 1–12 h after UVC irradiation and increased again at 24 h in both cell lines. Treatment with the selective COX-2 inhibitor, SC-791, during maximum expression of COX-2 induction, attenuated the UVC induced-growth inhibition in NIH 3T3 cells. In contrast, SC-791 treatment after UVC irradiation enhanced death of A431 cells. These data showed that the patterns of UVC-induced PGE2 secretion from NIH 3T3 cells and A431 cells were similar despite the differential profile in UVC-induced COX-2 up-regulation. Besides, COX-2 might play different roles in cellular response to UVC irradiation in various cell lines.
Keywords: ultraviolet C (UVC); cyclooxygenases-2 (COX-2); prostaglandin E2 (PGE2); NIH 3T3 cells; A431 cells

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MDPI and ACS Style

Tai, M.-H.; Weng, C.-H.; Mon, D.-P.; Hu, C.-Y.; Wu, M.-H. Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines. Int. J. Mol. Sci. 2012, 13, 4351-4366.

AMA Style

Tai M-H, Weng C-H, Mon D-P, Hu C-Y, Wu M-H. Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines. International Journal of Molecular Sciences. 2012; 13(4):4351-4366.

Chicago/Turabian Style

Tai, Ming-Hong; Weng, Chien-Hui; Mon, Dir-Pu; Hu, Chun-Yi; Wu, Ming-Hsiu. 2012. "Ultraviolet C Irradiation Induces Different Expression of Cyclooxygenase 2 in NIH 3T3 Cells and A431 Cells: The Roles of COX-2 Are Different in Various Cell Lines." Int. J. Mol. Sci. 13, no. 4: 4351-4366.

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