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• Chiang, T
• Tzeng, T
• Lin, H
• Cho, C
• Lin, F
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• Tzeng, T
• Lin, H
• Cho, C
• Lin, F
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• Tzeng, T
• Lin, H
• Cho, C
• Lin, F

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Int. J. Mol. Sci. 2012, 13(3), 2763-2768; doi:10.3390/ijms13032763

Article

Isolation and Characterization of Polymorphic Microsatellite Loci from Metapenaeopsis barbata Using PCR-Based Isolation of Microsatellite Arrays (PIMA)

Tzen-Yuh Chiang ^1,† , Tzong-Der Tzeng ^2,† , Hung-Du Lin ³ , Ching-Ju Cho ¹  and Feng-Jiau Lin ^1,*

¹ Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan ² Department of Leisure, Recreation and Tourism Management, Shu-Te University, Kaohsiung 824, Taiwan ³ The Affiliated School of National Tainan First Senior High School, Tainan 701, Taiwan ^† These authors contributed equally to this work.

* Author to whom correspondence should be addressed.

Received: 19 January 2012; in revised form: 19 February 2012 / Accepted: 21 February 2012 / Published: 1 March 2012

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

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Abstract: The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Overfishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as observed and expected heterozygosities in populations, ranging from 0.048 to 0.538, and 0.048 and 0.654, respectively, were detected. No deviation from Hardy–Weinberg expectations was detected at either locus. No significant linkage disequilibrium was detected in locus pairs. The polymorphic microsatellite loci will be useful for investigations of the genetic variation, population structure, and conservation genetics of this species.

Keywords: microsatellite; management; PIMA; RAPD-PCR enrichment; Metapenaeopsis barbata

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