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All-Trans Retinoic Acid Treatment Is Associated with Prohibitin Expression in Renal Interstitial Fibrosis Rats
Department of Pediatrics, The First Affiliated Hospital of GuangXi Medical University, NanNing 530021, China
* Author to whom correspondence should be addressed.
Received: 9 January 2012; in revised form: 23 February 2012 / Accepted: 23 February 2012 / Published: 1 March 2012
Abstract: This study was performed to investigate the association of prohibitin with renal interstitial fibrosis (RIF) lesion and to explore the association of all-trans retinoic acid (ATRA) treatment with prohibitin expression in RIF rats. Rats were divided into three groups: the sham operation group (SHO), the model group subjected to unilateral ureteral obstruction (UUO), and the model group treated with ATRA (GA). Renal tissues were collected at 14 and 28 days after surgery, and the relevant indicators were detected. In comparison with the SHO group, the RIF index in the UUO group was markedly elevated (p < 0.01), and the RIF index in the GA group was alleviated compared with that in the UUO group (p < 0.01). Compared with the SHO group, the expression of prohibitin (protein or mRNA) in the UUO group was significantly reduced (each p < 0.01). Prohibitin expression in the GA group was markedly increased when compared with that in the UUO (p < 0.01). The expression of TGF-β1 (protein and mRNA), protein expressions of Col-IV, fibronectin, α-SMA and cleaved Caspase-3, ROS generation and cell apoptosis index in the UUO group were markedly higher than those in the SHO group (all p < 0.01), and their expressions in the GA group were markedly down-regulated compared to those in the UUO group (all p < 0.01, respectively). The protein expression of prohibitin was negatively correlated with the RIF index, protein expression of TGF-β1, Col-IV, fibronectin, α-SMA or cleaved Caspase-3, ROS generation and the cell apoptosis index (each p < 0.01). In conclusion, lower expression of prohibitin is associated with the RIF, and ATRA treatment is associated with increased prohibitin, which can prevent the progression of RIF.
Keywords: all-trans retinoic acid; prohibitin, extracellular matrix; cell apoptosis; renal interstitial fibrosis; unilateral ureteral obstruction
A correction was published on 18 December 2012: http://www.mdpi.com/1422-0067/13/12/17295
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The authors wish to change Figure 2 of the paper published in IJMS . The positions of H1 and H2 in the previous article were reversed. These errors have been amended in an amended version of the manuscript, which is available from the International Journal of Molecular Sciences website. The authors and publisher apologize for the inconvenience.
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Zhou, T.-B.; Qin, Y.-H.; Li, Z.-Y.; Xu, H.-L.; Zhao, Y.-J.; Lei, F.-Y. All-Trans Retinoic Acid Treatment Is Associated with Prohibitin Expression in Renal Interstitial Fibrosis Rats. Int. J. Mol. Sci. 2012, 13, 2769-2782.
Zhou T-B, Qin Y-H, Li Z-Y, Xu H-L, Zhao Y-J, Lei F-Y. All-Trans Retinoic Acid Treatment Is Associated with Prohibitin Expression in Renal Interstitial Fibrosis Rats. International Journal of Molecular Sciences. 2012; 13(3):2769-2782.
Zhou, Tian-Biao; Qin, Yuan-Han; Li, Zheng-Yi; Xu, Hui-Ling; Zhao, Yan-Jun; Lei, Feng-Ying. 2012. "All-Trans Retinoic Acid Treatment Is Associated with Prohibitin Expression in Renal Interstitial Fibrosis Rats." Int. J. Mol. Sci. 13, no. 3: 2769-2782.