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Int. J. Mol. Sci. 2012, 13(2), 2459-2471; doi:10.3390/ijms13022459
Article

First Insights on Organic Cosolvent Effects on FhuA Wildtype and FhuA Δ1-159

 and *
Received: 6 January 2012; in revised form: 9 February 2012 / Accepted: 13 February 2012 / Published: 22 February 2012
(This article belongs to the Special Issue Applications of Circular Dichroism)
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Abstract: Circular dichroism (CD) and deconvolution were used to study the structural integrity of a “plugged” and an “open” FhuA transmembrane channel protein in the presence of varied concentrations of tetrahydrofuran (THF), ethanol (EtOH) and chloroform/methanol (C/M). FhuA is an Escherichia coli outer membrane protein (78.9 kDa) consisting of 22 β-sheets and an internal globular cork domain which acts as an iron transporter. FhuA and the deletion variant FhuA Δ1-159 showed comparable and remarkable resistance in the presence of THF (≤40 vol%) and EtOH (≤10 vol%). In C/M, significant differences in structural resistance were observed (FhuA stable ≤10 vol%; FhuA Δ1-159 ≤1 vol%). Deconvolution of CD-spectra for FhuA and FhuA Δ1-159 yielded β-sheet contents of 61 % (FhuA) and 58% (FhuA Δ1-159). Interestingly, FhuA and FhuA Δ1-159 had comparable β-sheet contents in the presence and absence of all three organic cosolvents. Additionally, precipitated FhuA and FhuA Δ1-159 (in 40 vol% C/M or 65 vol% THF) redissolved by supplementing the detergent n-octyl-oligo-oxyethylene (oPOE).
Keywords: secondary structure; circular dichroism; membrane protein; organic cosolvent; β-barrel; FhuA; precipitation; structural integrity secondary structure; circular dichroism; membrane protein; organic cosolvent; β-barrel; FhuA; precipitation; structural integrity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Tenne, S.-J.; Schwaneberg, U. First Insights on Organic Cosolvent Effects on FhuA Wildtype and FhuA Δ1-159. Int. J. Mol. Sci. 2012, 13, 2459-2471.

AMA Style

Tenne S-J, Schwaneberg U. First Insights on Organic Cosolvent Effects on FhuA Wildtype and FhuA Δ1-159. International Journal of Molecular Sciences. 2012; 13(2):2459-2471.

Chicago/Turabian Style

Tenne, Stefanie-Joana; Schwaneberg, Ulrich. 2012. "First Insights on Organic Cosolvent Effects on FhuA Wildtype and FhuA Δ1-159." Int. J. Mol. Sci. 13, no. 2: 2459-2471.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert