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Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan
Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung 402, Taiwan
* Author to whom correspondence should be addressed.
Received: 21 January 2012; in revised form: 22 February 2012 / Accepted: 24 February 2012 / Published: 12 March 2012
Abstract: Circular dichroism (CD) spectroscopy is an optical technique that measures the difference in the absorption of left and right circularly polarized light. This technique has been widely employed in the studies of nucleic acids structures and the use of it to monitor conformational polymorphism of DNA has grown tremendously in the past few decades. DNA may undergo conformational changes to B-form, A-form, Z-form, quadruplexes, triplexes and other structures as a result of the binding process to different compounds. Here we review the recent CD spectroscopic studies of the induction of DNA conformational changes by different ligands, which includes metal derivative complex of aureolic family drugs, actinomycin D, neomycin, cisplatin, and polyamine. It is clear that CD spectroscopy is extremely sensitive and relatively inexpensive, as compared with other techniques. These studies show that CD spectroscopy is a powerful technique to monitor DNA conformational changes resulting from drug binding and also shows its potential to be a drug-screening platform in the future.
Keywords: DNA structure; circular dichroism; conformational changes; DNA-binding drugs; thermodynamic parameters
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Chang, Y.-M.; Chen, C.-M.; Hou, M.-H. Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism. Int. J. Mol. Sci. 2012, 13, 3394-3413.
Chang Y-M, Chen C-M, Hou M-H. Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism. International Journal of Molecular Sciences. 2012; 13(3):3394-3413.
Chang, Yu-Ming; Chen, Cammy K.-M.; Hou, Ming-Hon. 2012. "Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism." Int. J. Mol. Sci. 13, no. 3: 3394-3413.