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Int. J. Mol. Sci. 2012, 13(2), 2239-2248; doi:10.3390/ijms13022239

Article

Nanosecond T-Jump Experiment in Poly(glutamic acid): A Circular Dichroism Study

Lucille Mendonça and François Hache*

Laboratory for Optics and Biosciences, Ecole Polytechnique/CNRS/INSERM, 91128 Palaiseau cedex, France

* Author to whom correspondence should be addressed.

Received: 5 January 2012; in revised form: 2 February 2012 / Accepted: 13 February 2012 / Published: 17 February 2012

(This article belongs to the Special Issue Applications of Circular Dichroism)

Download PDF Full-Text [215 KB, uploaded 17 February 2012 12:51 CET]

Abstract: Poly(glutamic acid) has been studied with a nanosecond T-jump experiment. A new experimental set-up based on the frequency-quadrupling of an 82 MHz Titanium-Sapphire laser allows rapid CD measurements to be performed. Combining time-resolved absorption and circular dichroism at 204 and 220 nm, we are able to measure precisely the unfolding relaxation time as well as the helical fraction evolution. We show that only CD at 220 nm is relevant to observe the unfolding of an alpha helix whereas no change is observed for CD at 204 nm. Conversely, both absorptions yield information on the dynamics of the process.

Keywords: circular dichroism; poly(glutamic acid); T-jump

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