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Int. J. Mol. Sci. 2012, 13(12), 16779-16784; doi:10.3390/ijms131216779
Article

Isolation and Characterization of Eleven Polymorphic Microsatellite Loci for the Valuable Medicinal Plant Dendrobium huoshanense and Cross-Species Amplification

1
, 2
, 1
, 1
, 1
 and 1,*
1 Key Laboratory of Molecular Evolution and Biodiversity and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Science, Anhui Normal University, Wuhu 241000, China 2 Engineering Technology Research Center of Plant Cell Engineering of Anhui Province, Lu'an 237012, China
* Author to whom correspondence should be addressed.
Received: 12 September 2012 / Revised: 14 November 2012 / Accepted: 29 November 2012 / Published: 7 December 2012
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Dendrobium huoshanense (Orchidaceae) is a perennial herb and a widely used medicinal plant in Traditional Chinese medicine (TCM) endemic to Huoshan County town in Anhui province in Southeast China. A microsatellite-enriched genomic DNA library of D. huoshanense was developed and screened to identify marker loci. Eleven polymorphic loci were isolated and analyzed by screening 25 individuals collected from a natural population. The number of alleles per locus ranged from 2 to 5. The observed and expected heterozygosities ranged from 0.227 to 0.818 and from 0.317 to 0.757, respectively. Two loci showed significant deviations from Hardy-Weinberg equilibrium and four of the pairwise comparisons of loci revealed linkage disequilibrium (p < 0.05). These microsatellite loci were cross-amplified for five congeneric species and seven loci can be amplified in all species. These simple sequence repeats (SSR) markers are useful in genetic studies of D. huoshanense and other related species and in conservation decision-making.
Keywords: Dendrobium huoshanense; medicinal plant; microsatellite; molecular marker; cross-amplification; population genetics Dendrobium huoshanense; medicinal plant; microsatellite; molecular marker; cross-amplification; population genetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, H.; Chen, N.-F.; Zheng, J.-Y.; Wang, W.-C.; Pei, Y.-Y.; Zhu, G.-P. Isolation and Characterization of Eleven Polymorphic Microsatellite Loci for the Valuable Medicinal Plant Dendrobium huoshanense and Cross-Species Amplification. Int. J. Mol. Sci. 2012, 13, 16779-16784.

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