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Int. J. Mol. Sci. 2012, 13(12), 16046-16052; doi:10.3390/ijms131216046
Article

Isolation and Characterization of Polymorphic Microsatellite Markers from the Chinese Medicinal Herb Atractylodes macrocephala (Asteraceae)

1,2
, 1,2
, 1,2
 and 1,2,*
1 Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, China 2 Laboratory of Systematic & Evolutionary Botany and Biodiversity, Institute of Plant Sciences, and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, China
* Author to whom correspondence should be addressed.
Received: 13 September 2012 / Revised: 16 October 2012 / Accepted: 26 October 2012 / Published: 28 November 2012
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

Atractylodes macrocephala Koidz. (Asteraceae) is an economically important Chinese medicinal herb. In this study, 15 polymorphic microsatellite markers were developed from A. macrocephala using the compound microsatellite marker technique. Levels of polymorphism within the 15 markers were assessed using 83 individuals from two wild and two cultivated populations in China. The number of alleles per locus ranged from 2 to 20, with an average of 9.9 alleles. Observed and expected heterozygosities ranged from 0.083 to 1.000 and from 0.097 to 0.938, respectively. These markers will be valuable for germplasm classification and identification, as well as for assessing the genetic diversity and spatial genetic structure among wild and cultivated populations of A. macrocephala.
Keywords: Atractylodes macrocephala; microsatellites; genetic diversity; Chinese medicinal herb Atractylodes macrocephala; microsatellites; genetic diversity; Chinese medicinal herb
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zheng, L.; Shao, Z.-D.; Wang, Z.-C.; Fu, C.-X. Isolation and Characterization of Polymorphic Microsatellite Markers from the Chinese Medicinal Herb Atractylodes macrocephala (Asteraceae). Int. J. Mol. Sci. 2012, 13, 16046-16052.

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