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Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads
Genetics Department, Biosciences Institute/Federal University of Rio Grande do Sul State – UFRGS, CEP 91501-970, Porto Alegre, RS, Brazil
Biological Sciences, Pantanal Campus/Federal University of Mato Grosso do Sul State – UFMS, CP 252, CEP 79304-902, Corumbá, MS, Brazil
Crop Sciences Department, Agronomy Center, Federal University of Santa Catarina State – UFSC, CP 476, CEP 88040-900, Florianópolis, SC, Brazil
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 13 October 2012; in revised form: 13 November 2012 / Accepted: 16 November 2012 / Published: 27 November 2012
Abstract: Microsatellite markers were isolated in Dyckia distachya, an endangered bromeliad from southern Brazil, which will be useful to assess the population genetic structure and reproductive success in introduced and natural populations of this species. Twenty microsatellite loci were developed from an enriched genomic library, and nine of these were amplified. The loci were characterized in 43 individuals from introduced and wild D. distachya populations. All nine loci were polymorphic, with four to ten alleles per locus. In an introduced population the observed and expected heterozygosities ranged from 0.136–0.667 and 0.543–0.877, respectively, while in a wild population it ranged from 0.000 to 0.895 and from 0.050 to 0.811, respectively. The development of these microsatellite markers will contribute to investigations of the reproductive potential and viability of introduced populations of D. distachya as well as the single known wild population. Cross-amplification in other Bromeliaceae species was successful, with high rates in four loci, demonstrating the applicability of these microsatellite markers in other taxa.
Keywords: Bromeliad; endemism; introduction; rheophytic species; SSR
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Zanella, C.M.; Janke, A.; Paggi, G.M.; Goetze, M.; Reis, M.S.; Bered, F. Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads. Int. J. Mol. Sci. 2012, 13, 15859-15866.
Zanella CM, Janke A, Paggi GM, Goetze M, Reis MS, Bered F. Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads. International Journal of Molecular Sciences. 2012; 13(12):15859-15866.
Zanella, Camila M.; Janke, Aline; Paggi, Gecele M.; Goetze, Márcia; Reis, Mauricio S.; Bered, Fernanda. 2012. "Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads." Int. J. Mol. Sci. 13, no. 12: 15859-15866.