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Int. J. Mol. Sci. 2012, 13(12), 15859-15866; doi:10.3390/ijms131215859
Article

Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads

1,†
, 1,†
, 2
, 1
, 3
 and 1,*
Received: 13 October 2012; in revised form: 13 November 2012 / Accepted: 16 November 2012 / Published: 27 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Microsatellite markers were isolated in Dyckia distachya, an endangered bromeliad from southern Brazil, which will be useful to assess the population genetic structure and reproductive success in introduced and natural populations of this species. Twenty microsatellite loci were developed from an enriched genomic library, and nine of these were amplified. The loci were characterized in 43 individuals from introduced and wild D. distachya populations. All nine loci were polymorphic, with four to ten alleles per locus. In an introduced population the observed and expected heterozygosities ranged from 0.136–0.667 and 0.543–0.877, respectively, while in a wild population it ranged from 0.000 to 0.895 and from 0.050 to 0.811, respectively. The development of these microsatellite markers will contribute to investigations of the reproductive potential and viability of introduced populations of D. distachya as well as the single known wild population. Cross-amplification in other Bromeliaceae species was successful, with high rates in four loci, demonstrating the applicability of these microsatellite markers in other taxa.
Keywords: Bromeliad; endemism; introduction; rheophytic species; SSR Bromeliad; endemism; introduction; rheophytic species; SSR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zanella, C.M.; Janke, A.; Paggi, G.M.; Goetze, M.; Reis, M.S.; Bered, F. Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads. Int. J. Mol. Sci. 2012, 13, 15859-15866.

AMA Style

Zanella CM, Janke A, Paggi GM, Goetze M, Reis MS, Bered F. Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads. International Journal of Molecular Sciences. 2012; 13(12):15859-15866.

Chicago/Turabian Style

Zanella, Camila M.; Janke, Aline; Paggi, Gecele M.; Goetze, Márcia; Reis, Mauricio S.; Bered, Fernanda. 2012. "Microsatellites in the Endangered Species Dyckia distachya (Bromeliaceae) and Cross-Amplification in Other Bromeliads." Int. J. Mol. Sci. 13, no. 12: 15859-15866.


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