Ginsenoside Rh2 Induces Human Hepatoma Cell Apoptosisvia Bax/Bak Triggered Cytochrome C Release and Caspase-9/Caspase-8 Activation
Abstract
:1. Introduction
2. Results
2.1. G-Rh2-Induced Apoptosis Is Mediated Via Caspase Activation
2.2. G-Rh2 Induces Caspase-9,-3,-7, and -8 Cleavages in SK-HEP-1 Cells
2.3. Apoptosis of G-Rh2-Induced SK-HEP-1 Cells Is Mediated through Cytochrome C Release and Depolarization of Mitochondrial Membrane Potential
2.4. G-Rh2-Induced Apoptosis in SK-HEP-1 Cells Is Associated with Mitochondrial Translocation of Bax and Bak
2.5. Caspase-8 Is Also Activated in G-Rh2-Treated Cells
3. Experimental Section
3.1. Materials
3.2. Cell Culture Conditions and Apoptosis Assays
3.3. Immuoblot Analysis
3.4. Caspase Assay
3.5. Preparation of Mitochondrial and Cytosolic Protein Extracts and Cytochrome C Release Assay
3.6. Depolarization Assay of Mitochondrial Membrane Potential by Staining with MitoCapture
3.7. RNA Interference
4. Discussion
5. Conclusions
Acknowledgments
References
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Sequence number | siRNA Duplex | |
---|---|---|
Sense (5′-3′) | Antisense (5′-3′) | |
1 | GCUGCUCUUCCGAAUUAAU | AUUAAUUCGGAAGAGCAGC |
2 | CCUGCUGGAUAUUUUCAUA | UAUGAAAAUAUCCAGCAGG |
3 | CAGAUCAGAAUUGAGGUCU | AGACCUCAAUUCUGAUCUG |
Negative control | CCUACGCCACCAAUUUCGU | ACGAAAUUGGUGGCGUAGG |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Guo, X.-X.; Guo, Q.; Li, Y.; Lee, S.K.; Wei, X.-N.; Jin, Y.-H. Ginsenoside Rh2 Induces Human Hepatoma Cell Apoptosisvia Bax/Bak Triggered Cytochrome C Release and Caspase-9/Caspase-8 Activation. Int. J. Mol. Sci. 2012, 13, 15523-15535. https://doi.org/10.3390/ijms131215523
Guo X-X, Guo Q, Li Y, Lee SK, Wei X-N, Jin Y-H. Ginsenoside Rh2 Induces Human Hepatoma Cell Apoptosisvia Bax/Bak Triggered Cytochrome C Release and Caspase-9/Caspase-8 Activation. International Journal of Molecular Sciences. 2012; 13(12):15523-15535. https://doi.org/10.3390/ijms131215523
Chicago/Turabian StyleGuo, Xiao-Xi, Qiao Guo, Yang Li, Seung Ki Lee, Xue-Ning Wei, and Ying-Hua Jin. 2012. "Ginsenoside Rh2 Induces Human Hepatoma Cell Apoptosisvia Bax/Bak Triggered Cytochrome C Release and Caspase-9/Caspase-8 Activation" International Journal of Molecular Sciences 13, no. 12: 15523-15535. https://doi.org/10.3390/ijms131215523