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Int. J. Mol. Sci. 2012, 13(11), 15227-15240; doi:10.3390/ijms131115227

Modification of Förster Resonance Energy Transfer Efficiencyat Interfaces

III. Institute of Physics – Biophysics, Georg August University, Friedrich-Hund-Platz 1, D-37077 Göttingen, Germany
Received: 7 October 2012 / Revised: 10 November 2012 / Accepted: 13 November 2012 / Published: 19 November 2012
(This article belongs to the Special Issue Förster Resonance Energy Transfer (FRET))
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Abstract

We present a theoretical study on the impact of an interface on the FRET efficiency of a surface-bound acceptor-donor system. The FRET efficiency can be modified by two effects. Firstly, the donor’s electromagnetic field at the acceptor’s position is changed due to the partial reflection of the donor’s field. Secondly, both the donor’s and the acceptor’s quantum yield of fluorescence can be changed due to the interface-induced enhancement of the radiative emission rate (Purcell effect). Numerical results for a FRET-pair at a glass-water interface are given.
Keywords: Förster Resonance Energy Transfer; single-molecule fluorescence; single-molecule electrodynamics Förster Resonance Energy Transfer; single-molecule fluorescence; single-molecule electrodynamics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Enderlein, J. Modification of Förster Resonance Energy Transfer Efficiencyat Interfaces. Int. J. Mol. Sci. 2012, 13, 15227-15240.

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