Int. J. Mol. Sci. 2012, 13(11), 14634-14647; doi:10.3390/ijms131114634
Article

Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-Induced Acute Liver Injury: An Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) Based Metabolomic Approach for the Pharmacodynamic Study of Traditional Chinese Medicines (TCMs)

1 School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 2 The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 3 The State Administration of Traditional Chinese Medicine (SATCM), Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 4 School of Life Science, Beijing Institute of Technology, Beijing 100081, China
* Author to whom correspondence should be addressed.
Received: 13 August 2012; in revised form: 12 October 2012 / Accepted: 1 November 2012 / Published: 9 November 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl4-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl4 injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
Keywords: Radix Paeoniae Alba; Radix Paeoniae Rubra; UPLC-MS; metabolomics; liver injury

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MDPI and ACS Style

Wang, R.; Xiong, A.-Z.; Teng, Z.-Q.; Yang, Q.-W.; Shi, Y.-H.; Yang, L. Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-Induced Acute Liver Injury: An Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) Based Metabolomic Approach for the Pharmacodynamic Study of Traditional Chinese Medicines (TCMs). Int. J. Mol. Sci. 2012, 13, 14634-14647.

AMA Style

Wang R, Xiong A-Z, Teng Z-Q, Yang Q-W, Shi Y-H, Yang L. Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-Induced Acute Liver Injury: An Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) Based Metabolomic Approach for the Pharmacodynamic Study of Traditional Chinese Medicines (TCMs). International Journal of Molecular Sciences. 2012; 13(11):14634-14647.

Chicago/Turabian Style

Wang, Rui; Xiong, Ai-Zhen; Teng, Zhong-Qiu; Yang, Qi-Wei; Shi, Yan-Hong; Yang, Li. 2012. "Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-Induced Acute Liver Injury: An Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) Based Metabolomic Approach for the Pharmacodynamic Study of Traditional Chinese Medicines (TCMs)." Int. J. Mol. Sci. 13, no. 11: 14634-14647.

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