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Int. J. Mol. Sci. 2012, 13(11), 14648-14666; doi:10.3390/ijms131114648
Review

An Advanced Electron Spin Resonance (ESR) Spin-Trapping and LC/(ESR)/MS Technique for the Study of Lipid Peroxidation

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Received: 29 September 2012; in revised form: 1 November 2012 / Accepted: 2 November 2012 / Published: 12 November 2012
(This article belongs to the Special Issue Advances in Free Radicals in Biology and Medicine)
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Abstract: There are two types of nutritionally important polyunsaturated fatty acids (PUFAs), namely ω-6s and ω-3s. PUFAs and their metabolites generated from lipid peroxidation via cyclooxygenase (COX) and lipoxygenase (LOX) are believed to be involved in a variety of physiological and pathological processes in the human body. Both COX- and LOX-catalyzed PUFA peroxidation are complex events that generate a series of radicals, which may then bind proteins, target DNA/RNA, and lead to a number of biological changes. However, due to the lack of an appropriate method, it was not possible until recently to identify the short-lived PUFA-derived radicals in COX-/LOX-catalyzed peroxidation. Failure to characterize free radicals during peroxidation has greatly restricted our knowledge about COX/LOX biology in human health. Here we review the development and refinement of combined ESR spin trapping and LC/ESR/MS to characterize PUFA-derived radicals formed from in vitro (cell-free) peroxidation. We also present the most recent approach for studying peroxidation in cells which allows us to directly assess the potential bioactivity of PUFA-derived free radicals. This advanced technique has resulted in a major breakthrough in radical structural characterization, as well as assessment of free radical-associated cell growth response, thereby greatly improving our knowledge of PUFAs, COX-/LOX-catalyzed lipid peroxidation, and their related biological consequences.
Keywords: COX- and LOX-catalyzed lipid peroxidation; ESR spin-trapping; free radicals from cellular peroxidation; human colon cancer HCA7 colony 29 cells; LC/ESR and LC/MS combined technique COX- and LOX-catalyzed lipid peroxidation; ESR spin-trapping; free radicals from cellular peroxidation; human colon cancer HCA7 colony 29 cells; LC/ESR and LC/MS combined technique
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Xu, Y.; Gu, Y.; Qian, S.Y. An Advanced Electron Spin Resonance (ESR) Spin-Trapping and LC/(ESR)/MS Technique for the Study of Lipid Peroxidation. Int. J. Mol. Sci. 2012, 13, 14648-14666.

AMA Style

Xu Y, Gu Y, Qian SY. An Advanced Electron Spin Resonance (ESR) Spin-Trapping and LC/(ESR)/MS Technique for the Study of Lipid Peroxidation. International Journal of Molecular Sciences. 2012; 13(11):14648-14666.

Chicago/Turabian Style

Xu, Yi; Gu, Yan; Qian, Steven Y. 2012. "An Advanced Electron Spin Resonance (ESR) Spin-Trapping and LC/(ESR)/MS Technique for the Study of Lipid Peroxidation." Int. J. Mol. Sci. 13, no. 11: 14648-14666.



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