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• Li, W
• Zhao, L
• Sun, Y
• Lei, F
• Wang, Z
• Gui, X
• Wang, H
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• Zhao, L
• Sun, Y
• Lei, F
• Wang, Z
• Gui, X
• Wang, H
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• Zhao, L
• Sun, Y
• Lei, F
• Wang, Z
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• Wang, H

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Int. J. Mol. Sci. 2012, 13(11), 14533-14544; doi:10.3390/ijms131114533

Article

Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design

Wei Li ^1,† , Li-Chun Zhao ^2,† , Yin-Shi Sun ³ , Feng-Jie Lei ¹ , Zi Wang ¹ , Xiong-Bin Gui ^2,*  and Hui Wang ^4,*

¹ College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China ² The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China ³ College of Agronomy, Shandong Agricultural University, Taian 271018, China ⁴ China-Japan Union Hospital, Jilin University, Changchun 130033, China ^† These two authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 16 July 2012; in revised form: 12 October 2012 / Accepted: 18 October 2012 / Published: 8 November 2012

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

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Abstract: In this work, pressurized liquid extraction (PLE) of three acetophenones (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for acetophenone extraction.

Keywords: pressurized liquid extraction; acetophenones; Cynanchum bungei Decne; Box-Behnken design; response surface methodology

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