Int. J. Mol. Sci. 2012, 13(11), 14533-14544; doi:10.3390/ijms131114533
Article

Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design

Received: 16 July 2012; in revised form: 12 October 2012 / Accepted: 18 October 2012 / Published: 8 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this work, pressurized liquid extraction (PLE) of three acetophenones (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for acetophenone extraction.
Keywords: pressurized liquid extraction; acetophenones; Cynanchum bungei Decne; Box-Behnken design; response surface methodology
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MDPI and ACS Style

Li, W.; Zhao, L.-C.; Sun, Y.-S.; Lei, F.-J.; Wang, Z.; Gui, X.-B.; Wang, H. Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design. Int. J. Mol. Sci. 2012, 13, 14533-14544.

AMA Style

Li W, Zhao L-C, Sun Y-S, Lei F-J, Wang Z, Gui X-B, Wang H. Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design. International Journal of Molecular Sciences. 2012; 13(11):14533-14544.

Chicago/Turabian Style

Li, Wei; Zhao, Li-Chun; Sun, Yin-Shi; Lei, Feng-Jie; Wang, Zi; Gui, Xiong-Bin; Wang, Hui. 2012. "Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design." Int. J. Mol. Sci. 13, no. 11: 14533-14544.

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