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Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
The Affiliated Ruikang Hospital, Guangxi University of Chinese Medicine, Nanning 530011, China
College of Agronomy, Shandong Agricultural University, Taian 271018, China
China-Japan Union Hospital, Jilin University, Changchun 130033, China
These two authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 16 July 2012; in revised form: 12 October 2012 / Accepted: 18 October 2012 / Published: 8 November 2012
Abstract: In this work, pressurized liquid extraction (PLE) of three acetophenones (4-hydroxyacetophenone, baishouwubenzophenone, and 2,4-dihydroxyacetophenone) from Cynanchum bungei (ACB) were investigated. The optimal conditions for extraction of ACB were obtained using a Box-Behnken design, consisting of 17 experimental points, as follows: Ethanol (100%) as the extraction solvent at a temperature of 120 °C and an extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 17 min. The extracted samples were analyzed by high-performance liquid chromatography using an UV detector. Under this optimal condition, the experimental values agreed with the predicted values by analysis of variance. The ACB extraction yield with optimal PLE was higher than that obtained by soxhlet extraction and heat-reflux extraction methods. The results suggest that the PLE method provides a good alternative for acetophenone extraction.
Keywords: pressurized liquid extraction; acetophenones; Cynanchum bungei Decne; Box-Behnken design; response surface methodology
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MDPI and ACS Style
Li, W.; Zhao, L.-C.; Sun, Y.-S.; Lei, F.-J.; Wang, Z.; Gui, X.-B.; Wang, H. Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design. Int. J. Mol. Sci. 2012, 13, 14533-14544.
Li W, Zhao L-C, Sun Y-S, Lei F-J, Wang Z, Gui X-B, Wang H. Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design. International Journal of Molecular Sciences. 2012; 13(11):14533-14544.
Li, Wei; Zhao, Li-Chun; Sun, Yin-Shi; Lei, Feng-Jie; Wang, Zi; Gui, Xiong-Bin; Wang, Hui. 2012. "Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design." Int. J. Mol. Sci. 13, no. 11: 14533-14544.