Next Article in Journal
Optimization of Pressurized Liquid Extraction of Three Major Acetophenones from Cynanchum bungei Using a Box-Behnken Design
Next Article in Special Issue
Role of Btg2 in the Progression of a PDGF-Induced Oligodendroglioma Model
Previous Article in Journal
Role of Key Residues at the Flavin Mononucleotide (FMN):Adenylyltransferase Catalytic Site of the Bifunctional Riboflavin Kinase/Flavin Adenine Dinucleotide (FAD) Synthetase from Corynebacterium ammoniagenes
Previous Article in Special Issue
Microenvironments and Cellular Characteristics in the Micro Tumor Cords of Malignant Solid Tumors
Int. J. Mol. Sci. 2012, 13(11), 14518-14532; doi:10.3390/ijms131114518
Article

Protein Profiling of Blood Samples from Patients with Hereditary Leiomyomatosis and Renal Cell Cancer by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

1,* , 2
,
1
,
3
,
4
 and
1
1 Department of Urology, Dokkyo Medical University, Tochigi 321-0293, Japan 2 Proteomics Research Unit, Division of Advanced Medicine, Medical Research Institute, Kanazawa Medical University, Ishikawa 920-0293, Japan 3 Department of Radiology, Dokkyo Medical University, Tochigi 321-0293, Japan 4 Department of Pathology, Dokkyo Medical University, Tochigi 321-0293, Japan
* Author to whom correspondence should be addressed.
Received: 31 July 2012 / Revised: 1 November 2012 / Accepted: 5 November 2012 / Published: 8 November 2012
(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))
View Full-Text   |   Download PDF [985 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Hereditary leiomyomatosis and renal cell cancer (HLRCC) is an extremely rare syndrome with autosomal dominant inheritance. HLRCC is characterized by a predisposition to leiomyomas of the skin and the uterus as well as renal cell carcinoma. The disease-related gene has been identified as fumarate hydratase (fumarase, FH), which encodes an enzyme involved in the mitochondrial tricarboxylic acid cycle. Protein profiling may give some insight into the molecular pathways of HLRCC. Therefore, we performed protein profiling of blood samples from HLRCC patients, their family members, and healthy volunteers, using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) coupled with IMAC-Cu chips. For hierarchical clustering analysis, we used the 45 peaks that revealed significant differences in single-marker analysis over the range from 1500 to 15,000 m/z. Heat map analysis based on the results of clustering distinguished the HLRCC kindred from non-HLRCC subjects with a sensitivity of 94% and a specificity of 90%. SELDI-TOF MS profiling of blood samples can be applied to identify patients with HLRCC and to assess specific molecular mechanisms involved in this condition.
Keywords: Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC); fumarate hydratase (FH); surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS); metastasis Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC); fumarate hydratase (FH); surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS); metastasis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Kamai, T.; Tomosugi, N.; Abe, H.; Kaji, Y.; Oyama, T.; Yoshida, K.-I. Protein Profiling of Blood Samples from Patients with Hereditary Leiomyomatosis and Renal Cell Cancer by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Int. J. Mol. Sci. 2012, 13, 14518-14532.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert