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Int. J. Mol. Sci. 2012, 13(11), 14251-14261; doi:10.3390/ijms131114251
Article

Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin

1,2
, 1
, 1
 and 1,2,*
Received: 11 September 2012; in revised form: 16 October 2012 / Accepted: 29 October 2012 / Published: 5 November 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL−1, and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin.
Keywords: β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cui, L.; Zhang, Z.-H.; Sun, E.; Jia, X.-B. Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin. Int. J. Mol. Sci. 2012, 13, 14251-14261.

AMA Style

Cui L, Zhang Z-H, Sun E, Jia X-B. Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin. International Journal of Molecular Sciences. 2012; 13(11):14251-14261.

Chicago/Turabian Style

Cui, Li; Zhang, Zhen-Hai; Sun, E; Jia, Xiao-Bin. 2012. "Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin." Int. J. Mol. Sci. 13, no. 11: 14251-14261.


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