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Int. J. Mol. Sci. 2012, 13(11), 14251-14261; doi:10.3390/ijms131114251

Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin

1 Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, Jiangsu, China 2 Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu, China
* Author to whom correspondence should be addressed.
Received: 11 September 2012 / Revised: 16 October 2012 / Accepted: 29 October 2012 / Published: 5 November 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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In the present paper, the effect of β-cyclodextrin (β-CD) inclusion complexation on the solubility and enzymatic hydrolysis of naringin was investigated. The inclusion complex of naringin/β-CD at the molar ratio of 1:1 was obtained by the dropping method and was characterized by differential scanning calorimetry. The solubility of naringin complexes in water at 37 ± 0.1 °C was 15 times greater than that of free naringin. Snailase-involved hydrolysis conditions were tested for the bioconversion of naringin into naringenin using the univariate experimental design. Naringin can be transformed into naringenin by snailase-involved hydrolysis. The optimum conditions for enzymatic hydrolysis were determined as follows: pH 5.0, temperature 37 °C, ratio of snailase/substrate 0.8, substrate concentration 20 mg·mL−1, and reaction time 12 h. Under the optimum conditions, the transforming rate of naringenin from naringin for inclusion complexes and free naringin was 98.7% and 56.2% respectively, suggesting that β-CD complexation can improve the aqueous solubility and consequently the enzymatic hydrolysis rate of naringin.
Keywords: β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin β-cyclodextrin; inclusion complex; enzymolysis; naringin; naringenin
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cui, L.; Zhang, Z.-H.; Sun, E.; Jia, X.-B. Effect of β-Cyclodextrin Complexation on Solubility and Enzymatic Conversion of Naringin. Int. J. Mol. Sci. 2012, 13, 14251-14261.

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