Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2012, 13(11), 14243-14250; doi:10.3390/ijms131114243
Article

Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species

1,* , 1
, 1
, 1
, 2
 and 1
Received: 5 September 2012; in revised form: 17 October 2012 / Accepted: 26 October 2012 / Published: 5 November 2012
View Full-Text   |   Download PDF [177 KB, uploaded 19 June 2014]
Abstract: Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.
Keywords: Abies; genetic conservation; mediterranean basin; nSSR; Pinaceae; population genetics Abies; genetic conservation; mediterranean basin; nSSR; Pinaceae; population genetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Sánchez-Robles, J.M.; Balao, F.; García-Castaño, J.L.; Terrab, A.; Navarro-Sampedro, L.; Talavera, S. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species. Int. J. Mol. Sci. 2012, 13, 14243-14250.

AMA Style

Sánchez-Robles JM, Balao F, García-Castaño JL, Terrab A, Navarro-Sampedro L, Talavera S. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species. International Journal of Molecular Sciences. 2012; 13(11):14243-14250.

Chicago/Turabian Style

Sánchez-Robles, Jose M.; Balao, Francisco; García-Castaño, Juan L.; Terrab, Anass; Navarro-Sampedro, Laura; Talavera, Salvador. 2012. "Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species." Int. J. Mol. Sci. 13, no. 11: 14243-14250.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert