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Int. J. Mol. Sci. 2012, 13(11), 14243-14250; doi:10.3390/ijms131114243

Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species

1
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Apdo. 1095, Sevilla E-41080, Spain
2
Centro de Investigación, Tecnología e Innovación (CITIUS), Universidad de Sevilla, Apdo. 1095, Sevilla E-41080, Spain
*
Author to whom correspondence should be addressed.
Received: 5 September 2012 / Revised: 17 October 2012 / Accepted: 26 October 2012 / Published: 5 November 2012
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Abstract

Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.
Keywords: Abies; genetic conservation; mediterranean basin; nSSR; Pinaceae; population genetics Abies; genetic conservation; mediterranean basin; nSSR; Pinaceae; population genetics
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sánchez-Robles, J.M.; Balao, F.; García-Castaño, J.L.; Terrab, A.; Navarro-Sampedro, L.; Talavera, S. Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species. Int. J. Mol. Sci. 2012, 13, 14243-14250.

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