Int. J. Mol. Sci. 2012, 13(11), 14262-14277; doi:10.3390/ijms131114262
Article

Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones

1 School of Food Science and Engineering, Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China 2 Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, China 3 Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, No.12 Zhongguancun South Street, Haidian District, Beijing 100081, China
* Authors to whom correspondence should be addressed.
Received: 4 September 2012; in revised form: 26 October 2012 / Accepted: 29 October 2012 / Published: 5 November 2012
(This article belongs to the Section Bioactives and Nutraceuticals)
PDF Full-text Download PDF Full-Text [382 KB, Updated Version, uploaded 8 November 2012 16:16 CET]
The original version is still available [476 KB, uploaded 5 November 2012 11:38 CET]
Abstract: The traditional method of gas chromatography-mass spectrometry for monosaccharide component analysis with pretreatment of acetylation is described with slight modifications and verified in detail in this paper. It was then successfully applied to the quantitative analysis of component monosaccharides in polysaccharides extracted from the pine cones. The results demonstrated that the three pine cone polysaccharides all consisted of ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose in different molar ratios. According to the recovery experiment, the described method was proved accurate and practical for the analysis of pine cone polysaccharides, meeting the need in the field of chemical analysis of Pinus plants. Furthermore; the chemical characteristics, such as neutral sugar, uronic acids, amino acids, molecular weights, and antioxidant activities of the polysaccharides were investigated by chemical and instrumental methods. The results showed that the chemical compositions of the polysaccharides differed from each other, especially in the content of neutral sugar and uronic acid. In the antioxidant assays, the polysaccharide fractions exhibited effective scavenging activities on ABTS radical and hydroxyl radical, with their antioxidant capabilities decreasing in the order of PKP > PAP > PSP. Therefore, although the polysaccharide fractions had little effect on superoxide radical scavenging, they still have potential to be developed as natural antioxidant agents in functional foods or medicine.
Keywords: Pinus; pine cone polysaccharide; GC-MS; antioxidant activity

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Xu, R.-B.; Yang, X.; Wang, J.; Zhao, H.-T.; Lu, W.-H.; Cui, J.; Cheng, C.-L.; Zou, P.; Huang, W.-W.; Wang, P.; Li, W.-J.; Hu, X.-L. Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones. Int. J. Mol. Sci. 2012, 13, 14262-14277.

AMA Style

Xu R-B, Yang X, Wang J, Zhao H-T, Lu W-H, Cui J, Cheng C-L, Zou P, Huang W-W, Wang P, Li W-J, Hu X-L. Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones. International Journal of Molecular Sciences. 2012; 13(11):14262-14277.

Chicago/Turabian Style

Xu, Ren-Bo; Yang, Xin; Wang, Jing; Zhao, Hai-Tian; Lu, Wei-Hong; Cui, Jie; Cheng, Cui-Lin; Zou, Pan; Huang, Wei-Wei; Wang, Pu; Li, Wen-Jing; Hu, Xing-Long. 2012. "Chemical Composition and Antioxidant Activities of Three Polysaccharide Fractions from Pine Cones." Int. J. Mol. Sci. 13, no. 11: 14262-14277.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert