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Int. J. Mol. Sci. 2012, 13(10), 13065-13078; doi:10.3390/ijms131013065
Article

Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L.) Osbeck) Peel and Their Antioxidant Activity

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College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China
* Author to whom correspondence should be addressed.
Received: 17 July 2012 / Revised: 11 September 2012 / Accepted: 25 September 2012 / Published: 12 October 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Supercritical carbon dioxide (SC-CO2) extraction of flavonoids from pomelo (Citrus grandis (L.) Osbeck) peel and their antioxidant activity were investigated. Box-Behnken design combined with response surface methodology was employed to maximize the extraction yield of flavonoids. Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be used to optimize the SC-CO2 extraction of flavonoids. The optimal conditions for obtaining the highest extraction yield of flavonoids from pomelo peel were a temperature of 80 °C, a pressure of 39 MPa and a static extraction time of 49 min in the presence of 85% ethanol as modifier. Under these conditions, the experimental yield was 2.37%, which matched positively with the value predicted by the model. Furthermore, flavonoids obtained by SC-CO2 extraction showed a higher scavenging activity on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals than those obtained by conventional solvent extraction (CSE). Therefore, SC-CO2 extraction can be considered as a suitable technique for the obtainment of flavonoids from pomelo peel.
Keywords: pomelo (Citrus grandis (L.) Osbeck) peel; flavonoids; supercritical carbon dioxide (SC-CO2) extraction; response surface methodology; antioxidant activity pomelo (Citrus grandis (L.) Osbeck) peel; flavonoids; supercritical carbon dioxide (SC-CO2) extraction; response surface methodology; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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He, J.-Z.; Shao, P.; Liu, J.-H.; Ru, Q.-M. Supercritical Carbon Dioxide Extraction of Flavonoids from Pomelo (Citrus grandis (L.) Osbeck) Peel and Their Antioxidant Activity. Int. J. Mol. Sci. 2012, 13, 13065-13078.

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