Int. J. Mol. Sci. 2012, 13(1), 683-697; doi:10.3390/ijms13010683
Review

Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies

David S. Kliger* email, Eefei Chenemail and Robert A. Goldbeckemail
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
* Author to whom correspondence should be addressed.
Received: 23 December 2011; in revised form: 5 January 2012 / Accepted: 5 January 2012 / Published: 10 January 2012
(This article belongs to the Special Issue Applications of Circular Dichroism)
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Abstract: Recent and ongoing developments in time-resolved spectroscopy have made it possible to monitor circular dichroism, magnetic circular dichroism, optical rotatory dispersion, and magnetic optical rotatory dispersion with nanosecond time resolution. These techniques have been applied to determine structural changes associated with the function of several proteins as well as to determine the nature of early events in protein folding. These studies have required new approaches in triggering protein reactions as well as the development of time-resolved techniques for polarization spectroscopies with sufficient time resolution and sensitivity to probe protein structural changes.
Keywords: circular dichroism; optical rotatory dispersion; magnetic circular dichroism; magnetic optical rotatory dispersion; heme proteins; cytochrome; phytochrome; polarization; quasi-null; ligand shuttle

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MDPI and ACS Style

Kliger, D.S.; Chen, E.; Goldbeck, R.A. Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies. Int. J. Mol. Sci. 2012, 13, 683-697.

AMA Style

Kliger DS, Chen E, Goldbeck RA. Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies. International Journal of Molecular Sciences. 2012; 13(1):683-697.

Chicago/Turabian Style

Kliger, David S.; Chen, Eefei; Goldbeck, Robert A. 2012. "Probing Kinetic Mechanisms of Protein Function and Folding with Time-Resolved Natural and Magnetic Chiroptical Spectroscopies." Int. J. Mol. Sci. 13, no. 1: 683-697.

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