Int. J. Mol. Sci. 2012, 13(1), 1258-1268; doi:10.3390/ijms13011258
Article

Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation

1,2email, 1,3email, 1,3, 1,3, 4email, 1,3,* email and 1,3,* email
Received: 9 October 2011; in revised form: 11 January 2012 / Accepted: 11 January 2012 / Published: 23 January 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Vascular inflammation is an important factor which can promote diabetic complications. In this study, the inhibitory effects of aqueous extract from Prunella vulgaris (APV) on high glucose (HG)-induced expression of cell adhesion molecules in human umbilical vein endothelial cells (HUVEC) are reported. APV decreased HG-induced expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. APV also dose-dependently inhibited HG-induced adhesion of HL-60 monocytic cells. APV suppressed p65 NF-κB activation in HG-treated cells. APV significantly inhibited the formation of intracellular reactive oxygen species (ROS). HG-stimulated HUVEC secreted gelatinases, however, APV inhibited it. APV induced Akt phosphorylation as well as activation of heme oxygenase-1 (HO-1), eNOS, and nuclear factor E2-related factor 2 (Nrf2), which may protect vascular inflammation caused by HG. In conclusion, APV exerts anti-inflammatory effect via inhibition of ROS/NF-κB pathway by inducing HO-1 and eNOS expression mediated by Nrf2, thereby suggesting that Prunella vulgaris may be a possible therapeutic approach to the inhibition of diabetic vascular diseases.
Keywords: Prunella vulgaris; inflammation; NF-κB; eNOS; Nrf2; atherosclerosis
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MDPI and ACS Style

Hwang, S.M.; Lee, Y.J.; Yoon, J.J.; Lee, S.M.; Kim, J.S.; Kang, D.G.; Lee, H.S. Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation. Int. J. Mol. Sci. 2012, 13, 1258-1268.

AMA Style

Hwang SM, Lee YJ, Yoon JJ, Lee SM, Kim JS, Kang DG, Lee HS. Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation. International Journal of Molecular Sciences. 2012; 13(1):1258-1268.

Chicago/Turabian Style

Hwang, Sun Mi; Lee, Yun Jung; Yoon, Jung Joo; Lee, So Min; Kim, Jin Sook; Kang, Dae Gill; Lee, Ho Sub. 2012. "Prunella vulgaris Suppresses HG-Induced Vascular Inflammation via Nrf2/HO-1/eNOS Activation." Int. J. Mol. Sci. 13, no. 1: 1258-1268.


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