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Int. J. Mol. Sci. 2011, 12(9), 5666-5671; doi:10.3390/ijms12095666
Article

Polymorphic Microsatellite Loci Isolated from the Squalidus argentatus Using PCR-Based Isolation of Microsatellite Arrays (PIMA)

1,†
,
2,†
,
1,†
,
3
,
1
,
1
 and
1,*
1 Laboratory of Fishes, Shanghai Ocean University, Shanghai, 201306, China 2 The Affiliated School of National Tainan First Senior High School, Tainan 701, Taiwan 3 National Museum of Marine Biology and Aquarium, Pingtung 900, Taiwan These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 28 June 2011 / Revised: 28 August 2011 / Accepted: 30 August 2011 / Published: 2 September 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Squalidus argentatus (Sauvage and Dabry de Thiersant 1874) is a small-sized freshwater fish which is distributed in Mainland China, Hainan Island and Taiwan. The populations of S. argentatus have dropped sharply probably due to overharvesting and water pollution recently. Eleven polymorphic microsatellite markers were developed for the cyprinid fish S. argentatus. These new markers were tested on 43 individuals collected from Yangtze River and Qiantang River. The number of alleles, observed and expected heterozygosity per locus, in two populations ranged from 3 to 14, from 0.333 to 0.954 and from 0.480 to 0.928, respectively. Only two loci are significantly deviated from Hardy–Weinberg expectations due to the heterozygote deficiency. No significant linkage disequilibrium was detected between the pairwise comparisons of these loci. These polymorphic microsatellite loci will enable us to study the genetic variation, population structure, and conservation genetics of this species in the future.
Keywords: microsatellite; conservation; PIMA; RAPD-PCR enrichment; Squalidus argentatus microsatellite; conservation; PIMA; RAPD-PCR enrichment; Squalidus argentatus
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sun, Y.; Lin, H.-D.; Tang, W.-Q.; Ju, Y.-M.; Liu, Z.-Z.; Liu, D.; Yang, J.-Q. Polymorphic Microsatellite Loci Isolated from the Squalidus argentatus Using PCR-Based Isolation of Microsatellite Arrays (PIMA). Int. J. Mol. Sci. 2011, 12, 5666-5671.

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