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Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora
Universidad Autónoma de Chapingo, Km. 38.5 Carretera México-Texcoco. Chapingo. Estado de México, CP 56230, Mexico
Universidad Politécnica de Pachuca, Km. 20 Carretera Pachuca Cd. Sahagún. Rancho Luna, Ex-Hacienda de Sta. Barbara, Municipio de Zempoala, Hidalgo, CP 42184, Mexico
Colegio de Postgraduados, Producción de Semillas, Km. 36.5 Carretera México-Texcoco. Edo. de México, CP 56230, Mexico
* Author to whom correspondence should be addressed.
Received: 21 July 2011; in revised form: 12 August 2011 / Accepted: 19 August 2011 / Published: 29 August 2011
Abstract: Stenocarpella maydis and Stenocarpella macrospora are the causal agents of ear rot in corn, which is one of the most destructive diseases in this crop worldwide. These fungi are important mycotoxin producers that cause different pathologies in farmed animals and represent an important risk for humans. In this work, 160 strains were isolated from soil of corn crops of which 10 showed antifungal activity against these phytopathogens, which, were identified as: Bacillus subtilis, Pseudomonas spp., Pseudomonas fluorescens, and Pantoea agglomerans by sequencing of 16S rRNA gene and the phylogenetic analysis. From cultures of each strain, extracellular filtrates were obtained and assayed to determine antifungal activity. The best filtrates were obtained in the stationary phase of B. subtilis cultures that were stable to the temperature and extreme pH values; in addition they did not show a cytotoxicity effect against brine shrimp and inhibited germination of conidia. The bacteria described in this work have the potential to be used in the control of white ear rot disease.
Keywords: Stenocarpella; biological control; Bacillus; Pseudomonas; Pantoea
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Petatán-Sagahón, I.; Anducho-Reyes, M.A.; Silva-Rojas, H.V.; Arana-Cuenca, A.; Tellez-Jurado, A.; Cárdenas-Álvarez, I.O.; Mercado-Flores, Y. Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. Int. J. Mol. Sci. 2011, 12, 5522-5537.
Petatán-Sagahón I, Anducho-Reyes MA, Silva-Rojas HV, Arana-Cuenca A, Tellez-Jurado A, Cárdenas-Álvarez IO, Mercado-Flores Y. Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. International Journal of Molecular Sciences. 2011; 12(9):5522-5537.
Petatán-Sagahón, Iván; Anducho-Reyes, Miguel Angel; Silva-Rojas, Hilda Victoria; Arana-Cuenca, Ainhoa; Tellez-Jurado, Alejandro; Cárdenas-Álvarez, Isabel Oyuki; Mercado-Flores, Yuridia. 2011. "Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora." Int. J. Mol. Sci. 12, no. 9: 5522-5537.