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Int. J. Mol. Sci. 2011, 12(10), 7265-7270; doi:10.3390/ijms12107265
Short Note

Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb

1,2,3,†
, 1,3,4,†
, 1
 and 1,3,*
Received: 26 September 2011; in revised form: 13 October 2011 / Accepted: 18 October 2011 / Published: 24 October 2011
(This article belongs to the Special Issue Advances in Molecular Ecology)
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Abstract: Erigeron breviscapus (Vant.) Hand.-Mazz. (Asteraceae) is a species endemic to southwestern China and an important traditional Chinese herb for cardiovascular and cerebral vessel diseases. Applying a modified biotin-streptavidin capture method, 11 microsatellite loci were discovered. Polymorphism of each locus was assessed in 24 individuals collected from five wild populations. The number of alleles per locus ranged from 2 to 7, with an average of 4.273. The observed (HO) and expected (HE) heterozygosities varied from 0.250 to 0.958 and from 0.337 to 0.786, respectively. Over half of these loci were successfully amplified in two congeneric species. The developed microsatellite markers will be useful for future population genetics and conservation studies, as well as accurate identification of different varieties.
Keywords: Erigeron breviscapus; microsatellite markers; polymorphism; population genetics Erigeron breviscapus; microsatellite markers; polymorphism; population genetics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, X.; Song, K.; Yang, J.; Yi, T. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb. Int. J. Mol. Sci. 2011, 12, 7265-7270.

AMA Style

Li X, Song K, Yang J, Yi T. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb. International Journal of Molecular Sciences. 2011; 12(10):7265-7270.

Chicago/Turabian Style

Li, Xiang; Song, Kexian; Yang, Junbo; Yi, Tingshuang. 2011. "Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb." Int. J. Mol. Sci. 12, no. 10: 7265-7270.


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