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Int. J. Mol. Sci. 2011, 12(10), 7265-7270; doi:10.3390/ijms12107265

Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb

1,2,3,†
,
1,3,4,†
,
1
and
1,3,*
1
Key laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
2
Graduate University of Chinese Academy of Sciences, Beijing 100049, China
3
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
4
Crop Research Institute of Sichuan Academy of Agricultural Sciences, Chengdu 610066, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 26 September 2011 / Revised: 13 October 2011 / Accepted: 18 October 2011 / Published: 24 October 2011
(This article belongs to the Special Issue Advances in Molecular Ecology)
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Abstract

Erigeron breviscapus (Vant.) Hand.-Mazz. (Asteraceae) is a species endemic to southwestern China and an important traditional Chinese herb for cardiovascular and cerebral vessel diseases. Applying a modified biotin-streptavidin capture method, 11 microsatellite loci were discovered. Polymorphism of each locus was assessed in 24 individuals collected from five wild populations. The number of alleles per locus ranged from 2 to 7, with an average of 4.273. The observed (HO) and expected (HE) heterozygosities varied from 0.250 to 0.958 and from 0.337 to 0.786, respectively. Over half of these loci were successfully amplified in two congeneric species. The developed microsatellite markers will be useful for future population genetics and conservation studies, as well as accurate identification of different varieties.
Keywords: Erigeron breviscapus; microsatellite markers; polymorphism; population genetics Erigeron breviscapus; microsatellite markers; polymorphism; population genetics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Li, X.; Song, K.; Yang, J.; Yi, T. Isolation and Characterization of 11 New Microsatellite Loci in Erigeron breviscapus (Asteraceae), an Important Chinese Traditional Herb. Int. J. Mol. Sci. 2011, 12, 7265-7270.

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