Next Article in Journal
Self-Assembly of Protein Monolayers Engineered for Improved Monoclonal Immunoglobulin G Binding
Next Article in Special Issue
Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange
Previous Article in Journal
Alert-QSAR. Implications for Electrophilic Theory of Chemical Carcinogenesis
Previous Article in Special Issue
A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations
Int. J. Mol. Sci. 2011, 12(8), 5135-5156; doi:10.3390/ijms12085135
Review

Enhancing Single Molecule Imaging in Optofluidics and Microfluidics

*  and
Received: 31 March 2011; in revised form: 23 May 2011 / Accepted: 25 July 2011 / Published: 12 August 2011
(This article belongs to the Special Issue Microfluidics)
View Full-Text   |   Download PDF [781 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Microfluidics and optofluidics have revolutionized high-throughput analysis and chemical synthesis over the past decade. Single molecule imaging has witnessed similar growth, due to its capacity to reveal heterogeneities at high spatial and temporal resolutions. However, both resolution types are dependent on the signal to noise ratio (SNR) of the image. In this paper, we review how the SNR can be enhanced in optofluidics and microfluidics. Starting with optofluidics, we outline integrated photonic structures that increase the signal emitted by single chromophores and minimize the excitation volume. Turning then to microfluidics, we review the compatible functionalization strategies that reduce noise stemming from non-specific interactions and architectures that minimize bleaching and blinking.
Keywords: optofluidics; microfluidics; single molecule; fluorescence; imaging; surface passivation; micro-fabrication; lab-on-a-chip optofluidics; microfluidics; single molecule; fluorescence; imaging; surface passivation; micro-fabrication; lab-on-a-chip
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Vasdekis, A.E.; Laporte, G.P. Enhancing Single Molecule Imaging in Optofluidics and Microfluidics. Int. J. Mol. Sci. 2011, 12, 5135-5156.

AMA Style

Vasdekis AE, Laporte GP. Enhancing Single Molecule Imaging in Optofluidics and Microfluidics. International Journal of Molecular Sciences. 2011; 12(8):5135-5156.

Chicago/Turabian Style

Vasdekis, Andreas E.; Laporte, Gregoire P.J. 2011. "Enhancing Single Molecule Imaging in Optofluidics and Microfluidics." Int. J. Mol. Sci. 12, no. 8: 5135-5156.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert