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Int. J. Mol. Sci. 2011, 12(8), 5135-5156; doi:10.3390/ijms12085135

Enhancing Single Molecule Imaging in Optofluidics and Microfluidics

Optics Laboratory, School of Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland
Author to whom correspondence should be addressed.
Received: 31 March 2011 / Revised: 23 May 2011 / Accepted: 25 July 2011 / Published: 12 August 2011
(This article belongs to the Special Issue Microfluidics)
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Microfluidics and optofluidics have revolutionized high-throughput analysis and chemical synthesis over the past decade. Single molecule imaging has witnessed similar growth, due to its capacity to reveal heterogeneities at high spatial and temporal resolutions. However, both resolution types are dependent on the signal to noise ratio (SNR) of the image. In this paper, we review how the SNR can be enhanced in optofluidics and microfluidics. Starting with optofluidics, we outline integrated photonic structures that increase the signal emitted by single chromophores and minimize the excitation volume. Turning then to microfluidics, we review the compatible functionalization strategies that reduce noise stemming from non-specific interactions and architectures that minimize bleaching and blinking.
Keywords: optofluidics; microfluidics; single molecule; fluorescence; imaging; surface passivation; micro-fabrication; lab-on-a-chip optofluidics; microfluidics; single molecule; fluorescence; imaging; surface passivation; micro-fabrication; lab-on-a-chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Vasdekis, A.E.; Laporte, G.P. Enhancing Single Molecule Imaging in Optofluidics and Microfluidics. Int. J. Mol. Sci. 2011, 12, 5135-5156.

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