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Enhancing Single Molecule Imaging in Optofluidics and Microfluidics
Optics Laboratory, School of Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland
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Received: 31 March 2011; in revised form: 23 May 2011 / Accepted: 25 July 2011 / Published: 12 August 2011
Abstract: Microfluidics and optofluidics have revolutionized high-throughput analysis and chemical synthesis over the past decade. Single molecule imaging has witnessed similar growth, due to its capacity to reveal heterogeneities at high spatial and temporal resolutions. However, both resolution types are dependent on the signal to noise ratio (SNR) of the image. In this paper, we review how the SNR can be enhanced in optofluidics and microfluidics. Starting with optofluidics, we outline integrated photonic structures that increase the signal emitted by single chromophores and minimize the excitation volume. Turning then to microfluidics, we review the compatible functionalization strategies that reduce noise stemming from non-specific interactions and architectures that minimize bleaching and blinking.
Keywords: optofluidics; microfluidics; single molecule; fluorescence; imaging; surface passivation; micro-fabrication; lab-on-a-chip
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Vasdekis, A.E.; Laporte, G.P. Enhancing Single Molecule Imaging in Optofluidics and Microfluidics. Int. J. Mol. Sci. 2011, 12, 5135-5156.
Vasdekis AE, Laporte GP. Enhancing Single Molecule Imaging in Optofluidics and Microfluidics. International Journal of Molecular Sciences. 2011; 12(8):5135-5156.
Vasdekis, Andreas E.; Laporte, Gregoire P.J. 2011. "Enhancing Single Molecule Imaging in Optofluidics and Microfluidics." Int. J. Mol. Sci. 12, no. 8: 5135-5156.