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Int. J. Mol. Sci. 2012, 13(1), 819-827; doi:10.3390/ijms13010819

Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange

1
Kanagawa Academy of Science and Technology, KSP East 310, 3-2-1 Sakado, Kawasaki 213-0012, Japan
2
Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo 101-0062, Japan
*
Author to whom correspondence should be addressed.
Received: 7 December 2011 / Revised: 6 January 2012 / Accepted: 9 January 2012 / Published: 13 January 2012
(This article belongs to the Special Issue Microfluidics)
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Abstract

We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time. View Full-Text
Keywords: microfluidic chip; Y-shape platform; medium exchange; environmental control; single cell; centrifugal force; on-chip cellomics microfluidic chip; Y-shape platform; medium exchange; environmental control; single cell; centrifugal force; on-chip cellomics
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hattori, A.; Yasuda, K. Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange. Int. J. Mol. Sci. 2012, 13, 819-827.

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