Int. J. Mol. Sci. 2012, 13(1), 819-827; doi:10.3390/ijms13010819
Article

Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange

1email and 1,2,* email
Received: 7 December 2011; in revised form: 6 January 2012 / Accepted: 9 January 2012 / Published: 13 January 2012
(This article belongs to the Special Issue Microfluidics)
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Abstract: We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.
Keywords: microfluidic chip; Y-shape platform; medium exchange; environmental control; single cell; centrifugal force; on-chip cellomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hattori, A.; Yasuda, K. Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange. Int. J. Mol. Sci. 2012, 13, 819-827.

AMA Style

Hattori A, Yasuda K. Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange. International Journal of Molecular Sciences. 2012; 13(1):819-827.

Chicago/Turabian Style

Hattori, Akihiro; Yasuda, Kenji. 2012. "Evaluation of a Centrifuged Double Y-Shape Microfluidic Platform for Simple Continuous Cell Environment Exchange." Int. J. Mol. Sci. 13, no. 1: 819-827.


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