Int. J. Mol. Sci. 2011, 12(7), 4271-4281; doi:10.3390/ijms12074271
Article

A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations

1,2email, 3,4email, 5, 6email, 7email and 1,2,* email
Received: 6 April 2011; in revised form: 16 June 2011 / Accepted: 20 June 2011 / Published: 1 July 2011
(This article belongs to the Special Issue Microfluidics)
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Abstract: In this paper, a rapid and simple method to determine the optimal temperature conditions for denaturant electrophoresis using a temperature-controlled on-chip capillary electrophoresis (CE) device is presented. Since on-chip CE operations including sample loading, injection and separation are carried out just by switching the electric field, we can repeat consecutive run-to-run CE operations on a single on-chip CE device by programming the voltage sequences. By utilizing the high-speed separation and the repeatability of the on-chip CE, a series of electrophoretic operations with different running temperatures can be implemented. Using separations of reaction products of single-stranded DNA (ssDNA) with a peptide nucleic acid (PNA) oligomer, the effectiveness of the presented method to determine the optimal temperature conditions required to discriminate a single-base substitution (SBS) between two different ssDNAs is demonstrated. It is shown that a single run for one temperature condition can be executed within 4 min, and the optimal temperature to discriminate the SBS could be successfully found using the present method.
Keywords: on-chip CE; denaturant electrophoresis; DNA separation; peptide nucleic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kaneda, S.; Ono, K.; Fukuba, T.; Nojima, T.; Yamamoto, T.; Fujii, T. A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations. Int. J. Mol. Sci. 2011, 12, 4271-4281.

AMA Style

Kaneda S, Ono K, Fukuba T, Nojima T, Yamamoto T, Fujii T. A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations. International Journal of Molecular Sciences. 2011; 12(7):4271-4281.

Chicago/Turabian Style

Kaneda, Shohei; Ono, Koichi; Fukuba, Tatsuhiro; Nojima, Takahiko; Yamamoto, Takatoki; Fujii, Teruo. 2011. "A Rapid Method for Optimizing Running Temperature of Electrophoresis through Repetitive On-Chip CE Operations." Int. J. Mol. Sci. 12, no. 7: 4271-4281.


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