Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2011, 12(8), 5060-5069; doi:10.3390/ijms12085060
Article

Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat

1,* , 2
, 1
 and 3
Received: 14 June 2011; in revised form: 21 July 2011 / Accepted: 4 August 2011 / Published: 8 August 2011
(This article belongs to the Section Bioactives and Nutraceuticals)
View Full-Text   |   Download PDF [215 KB, uploaded 19 June 2014]
Abstract: To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg−1, i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, laparotomized, and sutured without removing their ovaries. After 1 week of recovery from surgery, the OVX rats were randomly divided into three groups and orally treated with H2O (OVX group) or CHE (100 or 200 mg kg−1 daily) for 3 months. The sham-operated group (n = 8) was orally treated with H2O. After 3 months, the total body bone mineral density (BMD), bone mineral content (BMC), Bone biomechanical index, blood mineral levels and blood antioxidant enzymes activities were examined in sham-operated, ovariectomized and Cistanches Herba extract treated rats. Results showed that Cistanches Herba extract treatment significantly dose-dependently enhanced bone mineral density (BMD), bone mineral content (BMC), maximum load, displacement at maximum load, stress at maximum load, load at auto break, displacement at auto break, and stress at auto break, and blood antioxidant enzymes activities, decreased blood Ca, Zn and Cu levels compared to the OVX group. This experiment demonstrates that the administration of Cistanches Herba extract to ovariectomized rats reverses bone loss and prevents osteoporosis.
Keywords: ovariectomy; rat; bone; antioxidant; Cistanches Herba ovariectomy; rat; bone; antioxidant; Cistanches Herba
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Liang, H.; Yu, F.; Tong, Z.; Huang, Z. Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat. Int. J. Mol. Sci. 2011, 12, 5060-5069.

AMA Style

Liang H, Yu F, Tong Z, Huang Z. Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat. International Journal of Molecular Sciences. 2011; 12(8):5060-5069.

Chicago/Turabian Style

Liang, Haidong; Yu, Fang; Tong, Zhihong; Huang, Zaiguo. 2011. "Effect of Cistanches Herba Aqueous Extract on Bone Loss in Ovariectomized Rat." Int. J. Mol. Sci. 12, no. 8: 5060-5069.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert