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Controlled Delivery of Gentamicin Using Poly(3-hydroxybutyrate) Microspheres
Department of Applied and Molecular Biosciences, School of Life Sciences, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK
Department of Materials, Imperial College London Prince Consort Rd, London SW7 2AZ, UK
Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London 256 Gray’s Inn Road, London WC1X 8LD, UK
WCU Research Centre of Nanobiomedical Science, Dankook University, San#29, Anseo-dong, Dongnam-gu, Cheonan-si, Chungnam, 330-714, Korea
Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany
* Authors to whom correspondence should be addressed.
Received: 23 May 2011; in revised form: 27 June 2011 / Accepted: 27 June 2011 / Published: 4 July 2011
Abstract: Poly(3-hydroxybutyrate), P(3HB), produced from Bacillus cereus SPV using a simple glucose feeding strategy was used to fabricate P(3HB) microspheres using a solid-in-oil-water (s/o/w) technique. For this study, several parameters such as polymer concentration, surfactant and stirring rates were varied in order to determine their effect on microsphere characteristics. The average size of the microspheres was in the range of 2 µm to 1.54 µm with specific surface areas varying between 9.60 m2/g and 6.05 m2/g. Low stirring speed of 300 rpm produced slightly larger microspheres when compared to the smaller microspheres produced when the stirring velocity was increased to 800 rpm. The surface morphology of the microspheres after solvent evaporation appeared smooth when observed under SEM. Gentamicin was encapsulated within these P(3HB) microspheres and the release kinetics from the microspheres exhibiting the highest encapsulation efficiency, which was 48%, was investigated. The in vitro release of gentamicin was bimodal, an initial burst release was observed followed by a diffusion mediated sustained release. Biodegradable P(3HB) microspheres developed in this research has shown high potential to be used in various biomedical applications.
Keywords: Poly(3-hydroxybutyrate); microspheres; controlled drug delivery; gentamicin
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MDPI and ACS Style
Francis, L.; Meng, D.; Knowles, J.; Keshavarz, T.; Boccaccini, A.R.; Roy, I. Controlled Delivery of Gentamicin Using Poly(3-hydroxybutyrate) Microspheres. Int. J. Mol. Sci. 2011, 12, 4294-4314.
Francis L, Meng D, Knowles J, Keshavarz T, Boccaccini AR, Roy I. Controlled Delivery of Gentamicin Using Poly(3-hydroxybutyrate) Microspheres. International Journal of Molecular Sciences. 2011; 12(7):4294-4314.
Francis, Lydia; Meng, Decheng; Knowles, Jonathan; Keshavarz, Tajalli; Boccaccini, Aldo R.; Roy, Ipsita. 2011. "Controlled Delivery of Gentamicin Using Poly(3-hydroxybutyrate) Microspheres." Int. J. Mol. Sci. 12, no. 7: 4294-4314.