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Int. J. Mol. Sci. 2011, 12(7), 4282-4293; doi:10.3390/ijms12074282
Article

Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis

1,3,* , 2
,
1,2
 and
1,2
Received: 16 March 2011 / Revised: 3 June 2011 / Accepted: 13 June 2011 / Published: 4 July 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Catalase-loaded solid lipid nanoparticles (SLNs) were prepared by the double emulsion method (w/o/w) and solvent evaporation techniques, using acetone/methylene chloride (1:1) as an organic solvent, lecithin and triglyceride as oil phase and Poloxmer 188 as a surfactant. The optimized SLN was prepared by lecithin: triglyceride ratio (5%), 20-second + 30-second sonication, and 2% Poloxmer 188. The mean particle size of SLN was 296.0 ± 7.0 nm, polydispersity index range and zeta potential were 0.322–0.354 and −36.4 ± 0.6, respectively, and the encapsulation efficiency reached its maximum of 77.9 ± 1.56. Catalase distributed between the solid lipid and inner aqueous phase and gradually released from Poloxmer coated SLNs up to 20% within 20 h. Catalase-loaded SLN remained at 30% of H2O2-degrading activity after being incubated with Proteinase K for 24 h, while free catalase lost activity within 1 h.
Keywords: catalase; solid lipid nanoparticles; proteolysis; enzyme delivery catalase; solid lipid nanoparticles; proteolysis; enzyme delivery
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Qi, C.; Chen, Y.; Jing, Q.-Z.; Wang, X.-G. Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis. Int. J. Mol. Sci. 2011, 12, 4282-4293.

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