Int. J. Mol. Sci. 2011, 12(7), 4282-4293; doi:10.3390/ijms12074282
Article

Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis

1,3,* email, 2email, 1,2email and 1,2email
Received: 16 March 2011; in revised form: 3 June 2011 / Accepted: 13 June 2011 / Published: 4 July 2011
(This article belongs to the Section Material Sciences and Nanotechnology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Catalase-loaded solid lipid nanoparticles (SLNs) were prepared by the double emulsion method (w/o/w) and solvent evaporation techniques, using acetone/methylene chloride (1:1) as an organic solvent, lecithin and triglyceride as oil phase and Poloxmer 188 as a surfactant. The optimized SLN was prepared by lecithin: triglyceride ratio (5%), 20-second + 30-second sonication, and 2% Poloxmer 188. The mean particle size of SLN was 296.0 ± 7.0 nm, polydispersity index range and zeta potential were 0.322–0.354 and −36.4 ± 0.6, respectively, and the encapsulation efficiency reached its maximum of 77.9 ± 1.56. Catalase distributed between the solid lipid and inner aqueous phase and gradually released from Poloxmer coated SLNs up to 20% within 20 h. Catalase-loaded SLN remained at 30% of H2O2-degrading activity after being incubated with Proteinase K for 24 h, while free catalase lost activity within 1 h.
Keywords: catalase; solid lipid nanoparticles; proteolysis; enzyme delivery
PDF Full-text Download PDF Full-Text [300 KB, uploaded 19 June 2014 03:52 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Qi, C.; Chen, Y.; Jing, Q.-Z.; Wang, X.-G. Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis. Int. J. Mol. Sci. 2011, 12, 4282-4293.

AMA Style

Qi C, Chen Y, Jing Q-Z, Wang X-G. Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis. International Journal of Molecular Sciences. 2011; 12(7):4282-4293.

Chicago/Turabian Style

Qi, Ce; Chen, Yan; Jing, Qing-Zhe; Wang, Xing-Guo. 2011. "Preparation and Characterization of Catalase-Loaded Solid Lipid Nanoparticles Protecting Enzyme against Proteolysis." Int. J. Mol. Sci. 12, no. 7: 4282-4293.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert