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Int. J. Mol. Sci. 2011, 12(6), 3594-3605; doi:10.3390/ijms12063594
Article

Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip

1
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1
,
2
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1,*  and 2,*
1 Department of Vehicle Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan 2 Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
* Authors to whom correspondence should be addressed.
Received: 30 March 2011 / Revised: 11 May 2011 / Accepted: 25 May 2011 / Published: 3 June 2011
(This article belongs to the Special Issue Microfluidics)
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Abstract

This study presents an experimental and numerical investigation on the use of high-resolution injection techniques to deliver sample plugs within a capillary electrophoresis (CE) microchip. The CE microfluidic device was integrated into a U-shaped injection system and an expansion chamber located at the inlet of the separation channel, which can miniize the sample leakage effect and deliver a high-quality sample plug into the separation channel so that the detection performance of the device is enhanced. The proposed 45° U-shaped injection system was investigated using a sample of Rhodamine B dye. Meanwhile, the analysis of the current CE microfluidic chip was studied by considering the separation of Hae III digested φx-174 DNA samples. The experimental and numerical results indicate that the included 45° U-shaped injector completely eliminates the sample leakage and an expansion separation channel with an expansion ratio of 2.5 delivers a sample plug with a perfect detection shape and highest concentration intensity, hence enabling an optimal injection and separation performance.
Keywords: capillary electrophoresis microchip; injection system; expansion chamber capillary electrophoresis microchip; injection system; expansion chamber
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Chang, C.-L.; Leong, J.-C.; Hong, T.-F.; Wang, Y.-N.; Fu, L.-M. Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip. Int. J. Mol. Sci. 2011, 12, 3594-3605.

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