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Int. J. Mol. Sci. 2011, 12(6), 3594-3605; doi:10.3390/ijms12063594
Article

Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip

1
, 1
, 2
, 1,*  and 2,*
Received: 30 March 2011; in revised form: 11 May 2011 / Accepted: 25 May 2011 / Published: 3 June 2011
(This article belongs to the Special Issue Microfluidics)
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Abstract: This study presents an experimental and numerical investigation on the use of high-resolution injection techniques to deliver sample plugs within a capillary electrophoresis (CE) microchip. The CE microfluidic device was integrated into a U-shaped injection system and an expansion chamber located at the inlet of the separation channel, which can miniize the sample leakage effect and deliver a high-quality sample plug into the separation channel so that the detection performance of the device is enhanced. The proposed 45° U-shaped injection system was investigated using a sample of Rhodamine B dye. Meanwhile, the analysis of the current CE microfluidic chip was studied by considering the separation of Hae III digested φx-174 DNA samples. The experimental and numerical results indicate that the included 45° U-shaped injector completely eliminates the sample leakage and an expansion separation channel with an expansion ratio of 2.5 delivers a sample plug with a perfect detection shape and highest concentration intensity, hence enabling an optimal injection and separation performance.
Keywords: capillary electrophoresis microchip; injection system; expansion chamber capillary electrophoresis microchip; injection system; expansion chamber
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chang, C.-L.; Leong, J.-C.; Hong, T.-F.; Wang, Y.-N.; Fu, L.-M. Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip. Int. J. Mol. Sci. 2011, 12, 3594-3605.

AMA Style

Chang C-L, Leong J-C, Hong T-F, Wang Y-N, Fu L-M. Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip. International Journal of Molecular Sciences. 2011; 12(6):3594-3605.

Chicago/Turabian Style

Chang, Chin-Lung; Leong, Jik-Chang; Hong, Ting-Fu; Wang, Yao-Nan; Fu, Lung-Ming. 2011. "Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip." Int. J. Mol. Sci. 12, no. 6: 3594-3605.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert