Abstract: This study presents an experimental and numerical investigation on the use of high-resolution injection techniques to deliver sample plugs within a capillary electrophoresis (CE) microchip. The CE microfluidic device was integrated into a U-shaped injection system and an expansion chamber located at the inlet of the separation channel, which can miniize the sample leakage effect and deliver a high-quality sample plug into the separation channel so that the detection performance of the device is enhanced. The proposed 45° U-shaped injection system was investigated using a sample of Rhodamine B dye. Meanwhile, the analysis of the current CE microfluidic chip was studied by considering the separation of Hae III digested φx-174 DNA samples. The experimental and numerical results indicate that the included 45° U-shaped injector completely eliminates the sample leakage and an expansion separation channel with an expansion ratio of 2.5 delivers a sample plug with a perfect detection shape and highest concentration intensity, hence enabling an optimal injection and separation performance.
Keywords: capillary electrophoresis microchip; injection system; expansion chamber
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Chang, C.-L.; Leong, J.-C.; Hong, T.-F.; Wang, Y.-N.; Fu, L.-M. Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip. Int. J. Mol. Sci. 2011, 12, 3594-3605.
Chang C-L, Leong J-C, Hong T-F, Wang Y-N, Fu L-M. Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip. International Journal of Molecular Sciences. 2011; 12(6):3594-3605.
Chang, Chin-Lung; Leong, Jik-Chang; Hong, Ting-Fu; Wang, Yao-Nan; Fu, Lung-Ming. 2011. "Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip." Int. J. Mol. Sci. 12, no. 6: 3594-3605.