Next Article in Journal
Fully Automated On-Chip Imaging Flow Cytometry System with Disposable Contamination-Free Plastic Re-Cultivation Chip
Previous Article in Journal
Experimental and Numerical Analysis of High-Resolution Injection Technique for Capillary Electrophoresis Microchip
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2011, 12(6), 3606-3617; doi:10.3390/ijms12063606

Zebrafish Cyclin-Dependent Protein Kinase–Like 1 (zcdkl1): Identification and Functional Characterization

1
Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung 402, Taiwan
2
Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 402, Taiwan
3
School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 402, Taiwan
*
Author to whom correspondence should be addressed.
Received: 22 April 2011 / Revised: 19 May 2011 / Accepted: 22 May 2011 / Published: 3 June 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [865 KB, uploaded 19 June 2014]   |  

Abstract

The cyclin-dependent protein kinase family regulates a wide range of cellular functions such as cell cycle progression, differentiation, and apoptosis. In this study, we identified a zebrafish cyclin-dependent protein kinase-like 1 protein called zebrafish cdkl1 (zcdkl1), which shared a high degree of homology and conserved synteny with mammalian orthologs. zcdkl1 exhibited abilities for phosphorylation of myelin basic protein and histone H1. RT-PCR analysis revealed that zcdkl1 was expressed starting from fertilization and continuing thereafter. In adult tissues, zcdkl1 was predominantly detected in brain, ovary, and testis, and was expressed at low levels in other tissues. At 50% epiboly stage, zcdkl1 was widely expressed. At 12 to 48 h post-fertilization, zcdkl1 was predominantly expressed in the hypochord, the medial and lateral floor plate, and the pronephric duct. Interference of zcdkl1 expression resulted in abnormalities, such as brain and eye malformation, pericardial edema, and body axis curvature. Disruption of zcdkl1 reduced neurogenin-1 in the brain and sonic hedgehog expression in the floor plate region. These deformities were apparently rescued by co-injection of zcdkl1 mRNA. Findings of this study indicate that zcdkl1 plays an essential role in zebrafish development. View Full-Text
Keywords: cyclin dependent protein kinase-like 1 (cdkl1); expression pattern; floor plate; zebrafish cyclin dependent protein kinase-like 1 (cdkl1); expression pattern; floor plate; zebrafish
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hsu, L.-S.; Liang, C.-J.; Tseng, C.-Y.; Yeh, C.-W.; Tsai, J.-N. Zebrafish Cyclin-Dependent Protein Kinase–Like 1 (zcdkl1): Identification and Functional Characterization. Int. J. Mol. Sci. 2011, 12, 3606-3617.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top