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Int. J. Mol. Sci. 2011, 12(4), 2395-2407; doi:10.3390/ijms12042395
Article

Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Staphylococcus aureus Using Evolutionary Operation Factorial Design Technique

1
, 2
 and 1,*
1 School of Nano-Biotechnology & Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689–798, Korea 2 Korea Food Research Institute, Seongnam, Kyonggi, 463–746, Korea
* Author to whom correspondence should be addressed.
Received: 18 January 2011 / Revised: 7 February 2011 / Accepted: 28 March 2011 / Published: 6 April 2011
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

This study was undertaken to optimize extraction using evolutionary operation-factorial (EVOP) design technique to elicit the antibacterial activity of Perilla frutescens var. acuta leaf against Staphylococcus aureus ATCC6538. Higher antibacterial activity was achieved at higher extraction temperature and over a longer extraction time. Antibacterial activity was not affected by differentiation of the ethanol concentration in the extraction solvent. The maximum antibacterial activity of ethanolic extract of P. frutescens leaf against S. aureus was obtained at 75 °C (R = −0.7904**) extraction temperature, 24 h (R = −0.7273**) extraction time, and 45% (R = −0.0635) ethanol concentration. The population of S. aureus was decreased from 7.535 log CFU/mL in the initial set to 4.865 log CFU/mL in the third set by EVOP factorial design technique, as well as to 2.600 log CFU/mL by extraction with ethyl acetate. Further, the ethyl acetate extract revealed the highest phenolic contents (111.3 ± 8.6 mg% of dry sample) as compared to the other extracts. Also, the scanning electronic microscopic study of the ethanolic extract of P. frutescens revealed potential detrimental effect on the morphology of S. aureus.
Keywords: Perilla frutescens var. acuta leaf; EVOP-factorial design technique; antibacterial activity; Staphylococcus aureus ATCC6538 Perilla frutescens var. acuta leaf; EVOP-factorial design technique; antibacterial activity; Staphylococcus aureus ATCC6538
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kim, D.-H.; Kim, Y.-C.; Choi, U.-K. Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Staphylococcus aureus Using Evolutionary Operation Factorial Design Technique. Int. J. Mol. Sci. 2011, 12, 2395-2407.

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