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The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells
Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 150 Jimo Road, Shanghai 200120, China
Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China
Institutes of Biomedical Scienses, Fudan University, Shanghai 200032, China
* Authors to whom correspondence should be addressed.
Received: 31 December 2010; in revised form: 7 February 2011 / Accepted: 18 February 2011 / Published: 22 February 2011
Abstract: This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53.
Keywords: myocardial microvascular endothelial cell; G-SCF; Hypoxia inducible factor 1; p53 mRNA; HIF-1 mRNA
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MDPI and ACS Style
Li, J.; Zou, Y.; Ge, J.; Zhang, D.; Guan, A.; Wu, J.; Li, L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. Int. J. Mol. Sci. 2011, 12, 1306-1315.
Li J, Zou Y, Ge J, Zhang D, Guan A, Wu J, Li L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2011; 12(2):1306-1315.
Li, Jiming; Zou, Yunzeng; Ge, Junbo; Zhang, Daifu; Guan, Aili; Wu, Jian; Li, Lei. 2011. "The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells." Int. J. Mol. Sci. 12, no. 2: 1306-1315.