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Int. J. Mol. Sci. 2011, 12(2), 1306-1315; doi:10.3390/ijms12021306
Article

The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells

1
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 and 2,3
Received: 31 December 2010; in revised form: 7 February 2011 / Accepted: 18 February 2011 / Published: 22 February 2011
(This article belongs to the Section Molecular Pathology)
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Abstract: This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53.
Keywords: myocardial microvascular endothelial cell; G-SCF; Hypoxia inducible factor 1; p53 mRNA; HIF-1 mRNA myocardial microvascular endothelial cell; G-SCF; Hypoxia inducible factor 1; p53 mRNA; HIF-1 mRNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, J.; Zou, Y.; Ge, J.; Zhang, D.; Guan, A.; Wu, J.; Li, L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. Int. J. Mol. Sci. 2011, 12, 1306-1315.

AMA Style

Li J, Zou Y, Ge J, Zhang D, Guan A, Wu J, Li L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2011; 12(2):1306-1315.

Chicago/Turabian Style

Li, Jiming; Zou, Yunzeng; Ge, Junbo; Zhang, Daifu; Guan, Aili; Wu, Jian; Li, Lei. 2011. "The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells." Int. J. Mol. Sci. 12, no. 2: 1306-1315.


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