Int. J. Mol. Sci. 2011, 12(2), 1306-1315; doi:10.3390/ijms12021306
Article

The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells

1email, 2,3,* email, 2,3,* email, 1email, 2,3email, 2,3email and 2,3email
1 Department of Cardiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 150 Jimo Road, Shanghai 200120, China 2 Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China 3 Institutes of Biomedical Scienses, Fudan University, Shanghai 200032, China
* Authors to whom correspondence should be addressed.
Received: 31 December 2010; in revised form: 7 February 2011 / Accepted: 18 February 2011 / Published: 22 February 2011
(This article belongs to the Section Molecular Diagnostics)
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Abstract: This paper explores the effect of granulocyte colony-stimulating factor (G-CSF) on mouse myocardial microvascular endothelial cell (CMECs) proliferation. CMECs were harvested from C57/BL6 mice. CMECs were cultured in medium containing G-CSF (0 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL) for five days. Proliferative activity of CMECs was examined by CCK-8 method. Hypoxia inducible factor-1 (HIF-1) and p53 expression levels was determined from the mRNA obtained by reverse transcription polymerase chain reaction (RT-PCR). Results showed that the purity quotient of the CMECs, which were cultured by the method of modified myocardial tissue explant culture, was higher than 95%. Compared with control untreated cells, the proliferative activity of CMECs and the expression level of HIF-1 mRNA in these cells were enhanced by G-CSF treatment, whereas the expression level of p53 mRNA was markedly reduced. It may be concluded that G-CSF could promote the proliferative activity of CMECs, which might be mediated by upregulation of HIF-1 and downregulation of p53.
Keywords: myocardial microvascular endothelial cell; G-SCF; Hypoxia inducible factor 1; p53 mRNA; HIF-1 mRNA

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MDPI and ACS Style

Li, J.; Zou, Y.; Ge, J.; Zhang, D.; Guan, A.; Wu, J.; Li, L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. Int. J. Mol. Sci. 2011, 12, 1306-1315.

AMA Style

Li J, Zou Y, Ge J, Zhang D, Guan A, Wu J, Li L. The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2011; 12(2):1306-1315.

Chicago/Turabian Style

Li, Jiming; Zou, Yunzeng; Ge, Junbo; Zhang, Daifu; Guan, Aili; Wu, Jian; Li, Lei. 2011. "The Effects of G-CSF on Proliferation of Mouse Myocardial Microvascular Endothelial Cells." Int. J. Mol. Sci. 12, no. 2: 1306-1315.

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