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Int. J. Mol. Sci. 2011, 12(12), 8562-8574; doi:10.3390/ijms12128562
Article

Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats

1,* , 2
 and
3
1 Faculty of Medicine, Health and Molecular Sciences, James Cook University, Townsville 4811, Queensland, Australia 2 Division of Pathology, International Medical University, Kuala Lumpur 57000, Malaysia 3 Division of Human Biology, International Medical University, Kuala Lumpur 57000, Malaysia
* Author to whom correspondence should be addressed.
Received: 20 August 2011 / Revised: 18 October 2011 / Accepted: 20 October 2011 / Published: 29 November 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Angiogenesis factors are produced in response to hypoxic or ischemic insult at the site of pathology, which will cause neovascularization. Insulin like growth factor-1 (IGF-1) exerts potent proliferative, angiogenic and anti-apoptotic effects in target tissues. The present study was aimed to evaluate the effects of IGF-1 on circulating level of angiogenic cytokine interleukin-8 (IL-8), in experimentally-induced myocardial ischemia in rats. Male Sprague-Dawley rats were divided into control, IGF-1 treated (2 µg/kg/day subcutaneously, for 5 and 10 days), isoproterenol (ISO) treated (85 mg/kg, subcutaneously for two days) and ISO with IGF-1 treated (for 5 and 10 days). Heart weight, serum IGF-1, IL-8 and cardiac marker enzymes (CK-MB and LDH) were recorded after 5 and 10 days of treatment. Histopathological analyses of the myocardium were also done. There was a significant increase in serum cardiac markers with ISO treatment indicating myocardial infarction in rats. IGF-1 level increased significantly in ISO treated groups and the level of IGF-1 was significantly higher after 10 days of treatment. IL-8 level increased significantly after ISO treatment after 5 and 10 days and IGF-1 concurrent treatment to ISO rats had significantly increased IL-8 levels. Histopathologically, myocyte necrosis and nuclear pyknosis were reduced significantly in IGF-1 treated group and there were numerous areas of capillary sprouting suggestive of neovascularization in the myocardium. Thus, IGF-1 protects the ischemic myocardium with increased production of circulating angiogenic cytokine, IL-8 and increased angiogenesis.
Keywords: IGF-1; isoproterenol; myocardial ischemia; angiogenesis; interleukin-8 IGF-1; isoproterenol; myocardial ischemia; angiogenesis; interleukin-8
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Haleagrahara, N.; Chakravarthi, S.; Mathews, L. Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats. Int. J. Mol. Sci. 2011, 12, 8562-8574.

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