Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen
Abstract
:1. Introduction
2. Results
2.1. Expression of LeY Antigen in Cells Transfected with the hFUT Gene
2.2. Effects of Docetaxel on the Survival Rates of Cells
2.3. Docetaxel-Induced Apoptosis
2.4. Ultrastructure of Cells Examined by TEM
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Transfection of the Fucosyltransferase Gene
4.4. Immunocytochemical Assaying
4.5. Cell Viability Determined by MTT Assaying
4.6. Flow Cytometry
4.7. Transmission Electron Microscopy (TEM)
5. Conclusions
Acknowledgments
References
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Cell line | IC50 (μg/mL) | ta | Pa | tb | Pb |
---|---|---|---|---|---|
RMG-1-hFUT | 7.98 ± 1.31 | 2.88 | 0.05 | 3.34 | 0.03 |
RMG-1(-) | 4.60 ± 1.18 | 0.16 | 0.88 | ||
RMG-1 | 4.76 ± 1.42 |
Cell line | Apoptotic cells (%) | ta | Pa | tb | Pb |
---|---|---|---|---|---|
RMG-1-hFUT | 65.3 ± 2.1 | 7.42 | 0.006 | 9.50 | 0.002 |
RMG-1(-) | 83.7 ± 5.5 | 0.14 | 0.71 | ||
RMG-1 | 82.2 ± 6.4 |
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Zhang, F.; Liu, J.; Lin, B.; Liu, Q.; Zhao, Y.; Zhu, L.; Hao, Y.; Zhang, S.; Iwamori, M. Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen. Int. J. Mol. Sci. 2011, 12, 7323-7334. https://doi.org/10.3390/ijms12117323
Zhang F, Liu J, Lin B, Liu Q, Zhao Y, Zhu L, Hao Y, Zhang S, Iwamori M. Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen. International Journal of Molecular Sciences. 2011; 12(11):7323-7334. https://doi.org/10.3390/ijms12117323
Chicago/Turabian StyleZhang, Fan, Juanjuan Liu, Bei Lin, Qing Liu, Yue Zhao, Liancheng Zhu, Yingying Hao, Shulan Zhang, and Masao Iwamori. 2011. "Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen" International Journal of Molecular Sciences 12, no. 11: 7323-7334. https://doi.org/10.3390/ijms12117323