Int. J. Mol. Sci. 2011, 12(11), 7335-7351; doi:10.3390/ijms12117335
Article

A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device

Bilge Eker 1,†,§ email, Mahmut Deniz Yilmaz 2,†,|| email, Stefan Schlautmann 1 email, Johannes G. E. Gardeniers 1,* email and Jurriaan Huskens 2,* email
1 Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, University of Twente, 7500 AE, Enschede, The Netherlands 2 Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology, University of Twente, 7500 AE, Enschede, The Netherlands † These authors contributed equally to this work. § Present Address: EPFL-STI-IMT-LMIS4, Station 17, CH-1015 Lausanne, Switzerland; E-Mail: bilge.eker@epfl.ch. || Present Address: Northwestern University 633 Clark Street Evanston, IL 60208, USA; E-Mail: yilmazdnz@yahoo.com (M.D.Y.).
* Authors to whom correspondence should be addressed.
Received: 24 August 2011; in revised form: 19 October 2011 / Accepted: 20 October 2011 / Published: 26 October 2011
(This article belongs to the Special Issue Molecular Self-Assembly 2011)
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Abstract: A supramolecular platform based on self-assembled monolayers (SAMs) has been implemented in a microfluidic device. The system has been applied for the sensing of two different analyte types: biologically relevant phosphate anions and aromatic carboxylic acids, which are important for anthrax detection. A Eu(III)-EDTA complex was bound to β-cyclodextrin monolayers via orthogonal supramolecular host-guest interactions. The self-assembly of the Eu(III)-EDTA conjugate and naphthalene β-diketone as an antenna resulted in the formation of a highly luminescent lanthanide complex on the microchannel surface. Detection of different phosphate anions and aromatic carboxylic acids was demonstrated by monitoring the decrease in red emission following displacement of the antenna by the analyte. Among these analytes, adenosine triphosphate (ATP) and pyrophosphate, as well as dipicolinic acid (DPA) which is a biomarker for anthrax, showed a strong response. Parallel fabrication of five sensing SAMs in a single multichannel chip was performed, as a first demonstration of phosphate and carboxylic acid screening in a multiplexed format that allows a general detection platform for both analyte systems in a single test run with µM and nM detection sensitivity for ATP and DPA, respectively.
Keywords: microfluidic sensing device; phosphate and anthrax biomarker detection; lanthanide-based supramolecular sensing; surface-assisted ATP and DPA detection

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MDPI and ACS Style

Eker, B.; Yilmaz, M.D.; Schlautmann, S.; Gardeniers, J.G.E.; Huskens, J. A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device. Int. J. Mol. Sci. 2011, 12, 7335-7351.

AMA Style

Eker B, Yilmaz MD, Schlautmann S, Gardeniers JGE, Huskens J. A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device. International Journal of Molecular Sciences. 2011; 12(11):7335-7351.

Chicago/Turabian Style

Eker, Bilge; Yilmaz, Mahmut Deniz; Schlautmann, Stefan; Gardeniers, Johannes G. E.; Huskens, Jurriaan. 2011. "A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device." Int. J. Mol. Sci. 12, no. 11: 7335-7351.

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