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Int. J. Mol. Sci. 2011, 12(11), 7323-7334; doi:10.3390/ijms12117323
Article

Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen

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Received: 11 July 2011; in revised form: 13 October 2011 / Accepted: 17 October 2011 / Published: 26 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Epithelial carcinomas of the ovary exhibit the highest mortality rate among gynecologic malignancies. Studies found that the metabolism of glycolipids or carbohydrates is associated with acquirement of anticancer drug-resistance by cancer cells. This study was to characterize possible involvement of Lewis Y (LeY) antigen in the drug-resistance of cancer cells. We transfected the α1,2-fucosyltransferase gene into human ovarian carcinoma-derived RMG-1 cells and established RMG-1-hFUT cells with enhanced expression of LeY. We determined the effects of docetaxel on the survival of cells by MTT assaying and observed the apoptosis of cells in the presence of docetaxel by flow cytometric analysis and by transmission electron microscopy. Plasma membranes and intracellular granules in RMG-1-hFUT cells were stained with anti-LeY antibody, the intensity of the staining was higher than that in control cells. The RMG-1-hFUT cells exhibited higher resistance to docetaxel than the control cells with regard to the docetaxel concentration and time course. After treatment with 10 μg/mL docetaxel for 72 h, the control cells, but not RMG-1-hFUT, contained abundant positively stained cell debris due to disintegration of the cytoskeleton. On transmission electron microscopy, although the control cells treated with docetaxel as above showed the following morphology, i.e., absence of villi, cells shrunken in size, pyknosis, agglutinated chromatin and cell buds containing nuclei in the process of apoptosis, the RMG-1-hFUT cells showed only agglutinated chromatin and vacuoles in the cytoplasm. In summary, cells with enhanced expression of LeY were shown to acquire docetaxel-resistance, indicating the possible involvement of glycoconjugates in the drug-resistance.
Keywords: ovarian cancer; Lewis Y antigen; docetaxel; drug resistance ovarian cancer; Lewis Y antigen; docetaxel; drug resistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhang, F.; Liu, J.; Lin, B.; Liu, Q.; Zhao, Y.; Zhu, L.; Hao, Y.; Zhang, S.; Iwamori, M. Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen. Int. J. Mol. Sci. 2011, 12, 7323-7334.

AMA Style

Zhang F, Liu J, Lin B, Liu Q, Zhao Y, Zhu L, Hao Y, Zhang S, Iwamori M. Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen. International Journal of Molecular Sciences. 2011; 12(11):7323-7334.

Chicago/Turabian Style

Zhang, Fan; Liu, Juanjuan; Lin, Bei; Liu, Qing; Zhao, Yue; Zhu, Liancheng; Hao, Yingying; Zhang, Shulan; Iwamori, Masao. 2011. "Increase in Docetaxel-Resistance of Ovarian Carcinoma-Derived RMG-1 Cells with Enhanced Expression of Lewis Y Antigen." Int. J. Mol. Sci. 12, no. 11: 7323-7334.


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