Int. J. Mol. Sci. 2011, 12(10), 6619-6634; doi:10.3390/ijms12106619

Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells

1email, 2email and 3,* email
Received: 16 August 2011; in revised form: 20 September 2011 / Accepted: 23 September 2011 / Published: 10 October 2011
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Serotonin (5-HT) is a monoamine neurotransmitter and plays important roles in several of the human body’s systems. Known as a primary target for psychoactive drug development, the 5-HT transporter (5-HTT, SERT) plays a critical role in the regulation of serotonergic function by reuptaking 5-HT. The allelic variation of 5-HTT expression is caused by functional gene promoter polymorphism with two principal variant alleles, 5-HTT gene-linked polymorphic region (5-HTTLPR). It has been demonstrated that 5-HTTLPR is associated with numerous neuropsychiatric disorders. The functional roles of 5-HTTLPR have been reported in human choriocarcinoma (JAR), lymphoblast and raphe cells. To date, the significance of 5-HTTLPR in gastrointestinal tract-derived cells has never been elucidated. Thus, the impact of 5-HTTLPR on 5-HTT transcription was studied in SW480 human colon carcinoma cells, which were shown to express 5-HTT. We found 42-bp fragment in long (L) allele as compared to short (S) allele, and this allelic difference resulted in 2-fold higher transcriptional efficiency of L allele (P < 0.05) as demonstrated using a functional reporter gene assay. Nevertheless, the transcriptional effect of estrogen and glucocorticoid on 5-HTT expression via 5-HTTLPR was not found in this cell line. Our study was the first to demonstrate the molecular role of this allelic variation in gastrointestinal tract cells.
Keywords: serotonin transporter; allelic variation; transcriptional regulation; promoter; hormonal effect; gastrointestinal tract cells
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MDPI and ACS Style

Prasansuklab, A.; Poovorawan, Y.; Tencomnao, T. Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells. Int. J. Mol. Sci. 2011, 12, 6619-6634.

AMA Style

Prasansuklab A, Poovorawan Y, Tencomnao T. Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells. International Journal of Molecular Sciences. 2011; 12(10):6619-6634.

Chicago/Turabian Style

Prasansuklab, Anchalee; Poovorawan, Yong; Tencomnao, Tewin. 2011. "Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells." Int. J. Mol. Sci. 12, no. 10: 6619-6634.

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