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Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells
AbstractSerotonin (5-HT) is a monoamine neurotransmitter and plays important roles in several of the human body’s systems. Known as a primary target for psychoactive drug development, the 5-HT transporter (5-HTT, SERT) plays a critical role in the regulation of serotonergic function by reuptaking 5-HT. The allelic variation of 5-HTT expression is caused by functional gene promoter polymorphism with two principal variant alleles, 5-HTT gene-linked polymorphic region (5-HTTLPR). It has been demonstrated that 5-HTTLPR is associated with numerous neuropsychiatric disorders. The functional roles of 5-HTTLPR have been reported in human choriocarcinoma (JAR), lymphoblast and raphe cells. To date, the significance of 5-HTTLPR in gastrointestinal tract-derived cells has never been elucidated. Thus, the impact of 5-HTTLPR on 5-HTT transcription was studied in SW480 human colon carcinoma cells, which were shown to express 5-HTT. We found 42-bp fragment in long (L) allele as compared to short (S) allele, and this allelic difference resulted in 2-fold higher transcriptional efficiency of L allele (P < 0.05) as demonstrated using a functional reporter gene assay. Nevertheless, the transcriptional effect of estrogen and glucocorticoid on 5-HTT expression via 5-HTTLPR was not found in this cell line. Our study was the first to demonstrate the molecular role of this allelic variation in gastrointestinal tract cells.
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Prasansuklab, A.; Poovorawan, Y.; Tencomnao, T. Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells. Int. J. Mol. Sci. 2011, 12, 6619-6634.View more citation formats
Prasansuklab A, Poovorawan Y, Tencomnao T. Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells. International Journal of Molecular Sciences. 2011; 12(10):6619-6634.Chicago/Turabian Style
Prasansuklab, Anchalee; Poovorawan, Yong; Tencomnao, Tewin. 2011. "Modulation of Human Serotonin Transporter Expression by 5-HTTLPR in Colon Cells." Int. J. Mol. Sci. 12, no. 10: 6619-6634.