Next Article in Journal
Isolation and Characterization of Microsatellite Loci in the Asian Rice Gall Midge (Orseolia oryzae) (Diptera: Cecidomyiidae)
Previous Article in Journal
Susceptibility of Mycobacterium immunogenum and Pseudomonas fluorescens to Formaldehyde and Non-Formaldehyde Biocides in Semi-Synthetic Metalworking Fluids
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2011, 12(1), 742-754; doi:10.3390/ijms12010742

Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons

College of Fisheries, Huazhong Agricultural University, Wuhan 430070, Hubei, China
Department of Pharmacy, The Affiliated Xiangfan Hospital of TongJi Medical College of Huazhong University of Science & Technology, Xiangfan 441021, Hubei, China
Department of Pharmacology, Medical College, Xianning University, Xianning 437100, Hubei, China
Department of Osteopaedics, Center Hospital of Xianning, Xianning 437100, Hubei, China
Author to whom correspondence should be addressed.
Received: 22 December 2010 / Revised: 30 December 2010 / Accepted: 4 January 2011 / Published: 20 January 2011
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [489 KB, uploaded 19 June 2014]   |  


Green tea has been receiving considerable attention as a possible neuroprotective agent against neurodegenerative disease. Epigallocatechin-3-gallate (EGCG) is the major compound of green tea. Calcium signaling has profound effects on almost all aspects of neuronal function. Using digital calcium imaging and patch-clamp technique, we determined the effects of EGCG on Ca2+ signals in hippocampal neurons. The results indicated that EGCG caused a dose-dependent increase in intracellular Ca2+ ([Ca2+]i). This [Ca2+]i increase was blocked by depleting intracellular Ca2+ stores with the endoplasmic reticulum Ca2+ pump inhibitor thapsigargin and cyclopiazonic acid. Furthermore, EGCG-stimulated increase in [Ca2+]i was abolished following treatment with a PLC inhibitor. However, EGCG inhibited high-voltage activated Ca2+ currents (IHVA) and NMDA-induced inward currents (INMDA). These data suggest that EGCG triggers a cascade of events: it activates phospholipase C (PLC), mobilizes intracellular Ca2+ stores, raises the cytosolic Ca2+ levels, and inhibits the VGCC and NMDA receptors-mediated Ca2+ influx through a process that remains to be determined. View Full-Text
Keywords: EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron EGCG; calcium imaging; intracellular Ca2+; phopholipase C; hippocampal neuron

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, J.-H.; Cheng, J.; Li, C.-R.; Ye, M.; Ma, Z.; Cai, F. Modulation of Ca2+ Signals by Epigallocatechin-3-gallate(EGCG) in Cultured Rat Hippocampal Neurons. Int. J. Mol. Sci. 2011, 12, 742-754.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top