Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging
Abstract
:1. Introduction
2. Results and Discussion
2.1. Comparison of Particle Internalization and Internalization Kinetics in Vitro
2.2. Long-Term Stability of Labeling in Vitro
2.3. Cellular Toxicity and Plasma Binding
2.4. Cellular Localization of Absorbed Iron Oxide Nanoparticles
2.5. MRI Visualization in Vitro
3. Experimental Section
3.1. Cell Culture, Contrast Agents and Cell Labeling
3.2. Quantification of Iron
3.3. Cell-Toxicity
3.4. Visualization by Prussian Blue Staining and Electron Microscopy
3.5. Magnetic Resonance Imaging (MRI)
4. Conclusions
Acknowledgements
References
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Particle | Core | Coating | Hydrodynamic Diameter | Supplier |
---|---|---|---|---|
Resovist | Maghemite/magnetite | Carboxydextran | 60 nm | Schering (Berlin, Germany) |
B102 | Maghemite/magnetite | Carboxymethyldextran | 60 nm | Dr. Norbert Buske (Berlin, Germany) |
Non-coated Fe3O4 | Magnetite | None | 10 nm | Dr. Norbert Buske (Berlin, Germany) |
Microspheres (M310) | 30–60% Ferrite | Polystyrene | 310 nm | Merck Estapor (Fontenay Sous Bois, France) |
Microspheres (M850) | 55–65% Ferrite | Polystyrene | 850 nm | Merck Estapor (Fontenay Sous Bois, France) |
Nanomag20 (N20) | Magnetite | Dextran | 20 nm | Micromod (Rostock, Germany) |
Nanomag70 (N70) | Maghemite | Dextran | 70 nm | Micromod (Rostock, Germany) |
Nanomag100 (N100) | Maghemite | Dextran | 100 nm | Micromod (Rostock, Germany) |
© 2011 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Schlorf, T.; Meincke, M.; Kossel, E.; Glüer, C.-C.; Jansen, O.; Mentlein, R. Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging. Int. J. Mol. Sci. 2011, 12, 12-23. https://doi.org/10.3390/ijms12010012
Schlorf T, Meincke M, Kossel E, Glüer C-C, Jansen O, Mentlein R. Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging. International Journal of Molecular Sciences. 2011; 12(1):12-23. https://doi.org/10.3390/ijms12010012
Chicago/Turabian StyleSchlorf, Thomas, Manuela Meincke, Elke Kossel, Claus-Christian Glüer, Olav Jansen, and Rolf Mentlein. 2011. "Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging" International Journal of Molecular Sciences 12, no. 1: 12-23. https://doi.org/10.3390/ijms12010012