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Int. J. Mol. Sci. 2011, 12(1), 12-23; doi:10.3390/ijms12010012

Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging

1
Department of Anatomy, Christian-Albrechts-University Kiel, Otto-Hahn-Platz 8, 24118 Kiel, Germany
2
Department of Radiology, Division of Medical Physics, University Clinic Schleswig-Holstein, Campus Kiel, Arnold Heller Straße 9, 24105 Kiel, Germany
3
Department of Neuroradiology, University Clinic Schleswig-Holstein, Campus Kiel, Schittenhelmstraße 10, 24105 Kiel, Germany
*
Author to whom correspondence should be addressed.
Received: 14 October 2010 / Revised: 9 December 2010 / Accepted: 21 December 2010 / Published: 23 December 2010
(This article belongs to the Special Issue Magnetic Nanoparticles)
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Abstract

Superparamagnetic iron-oxide particles (SPIO) are used in different ways as contrast agents for magnetic resonance imaging (MRI): Particles with high nonspecific uptake are required for unspecific labeling of phagocytic cells whereas those that target specific molecules need to have very low unspecific cellular uptake. We compared iron-oxide particles with different core materials (magnetite, maghemite), different coatings (none, dextran, carboxydextran, polystyrene) and different hydrodynamic diameters (20–850 nm) for internalization kinetics, release of internalized particles, toxicity, localization of particles and ability to generate contrast in MRI. Particle uptake was investigated with U118 glioma cells und human umbilical vein endothelial cells (HUVEC), which exhibit different phagocytic properties. In both cell types, the contrast agents Resovist, B102, non-coated Fe3O4 particles and microspheres were better internalized than dextran-coated Nanomag particles. SPIO uptake into the cells increased with particle/iron concentrations. Maximum intracellular accumulation of iron particles was observed between 24 h to 36 h of exposure. Most particles were retained in the cells for at least two weeks, were deeply internalized, and only few remained adsorbed at the cell surface. Internalized particles clustered in the cytosol of the cells. Furthermore, all particles showed a low toxicity. By MRI, monolayers consisting of 5000 Resovist-labeled cells could easily be visualized. Thus, for unspecific cell labeling, Resovist and microspheres show the highest potential, whereas Nanomag particles are promising contrast agents for target-specific labeling.
Keywords: iron oxide nanoparticles; magnetic resonance imaging (MRI); toxicity; uptake kinetics; cellular localization; iron staining; electron microscopy iron oxide nanoparticles; magnetic resonance imaging (MRI); toxicity; uptake kinetics; cellular localization; iron staining; electron microscopy
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MDPI and ACS Style

Schlorf, T.; Meincke, M.; Kossel, E.; Glüer, C.-C.; Jansen, O.; Mentlein, R. Biological Properties of Iron Oxide Nanoparticles for Cellular and Molecular Magnetic Resonance Imaging. Int. J. Mol. Sci. 2011, 12, 12-23.

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