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The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand
Department of Pharmaceutical Technology and Drug Delivery System Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkla 90110, Thailand
* Author to whom correspondence should be addressed.
Received: 16 April 2010; in revised form: 12 May 2010 / Accepted: 13 May 2010 / Published: 20 May 2010
Abstract: Silk sericin (SS) can accelerate cell proliferation and attachment; however, SS can be extracted by various methods, which result in SS exhibiting different physical and biological properties. We found that SS produced from various extraction methods has different molecular weights, zeta potential, particle size and amino acid content. The MTT assay indicated that SS from all extraction methods had no toxicity to mouse fibroblast cells at concentrations up to 40 μg/mL after 24 h incubation, but SS obtained from some extraction methods can be toxic at higher concentrations. Heat-degraded SS was the least toxic to cells and activated the highest collagen production, while urea-extracted SS showed the lowest cell viability and collagen production. SS from urea extraction was severely harmful to cells at concentrations higher than 100 μg/mL. SS from all extraction methods could still promote collagen production in a concentration-dependent manner, even at high concentrations that are toxic to cells.
Keywords: sericin; cell viability; collagen; extraction; concentration
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MDPI and ACS Style
Aramwit, P.; Kanokpanont, S.; Nakpheng, T.; Srichana, T. The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production. Int. J. Mol. Sci. 2010, 11, 2200-2211.
Aramwit P, Kanokpanont S, Nakpheng T, Srichana T. The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production. International Journal of Molecular Sciences. 2010; 11(5):2200-2211.
Aramwit, Pornanong; Kanokpanont, Sorada; Nakpheng, Titpawan; Srichana, Teerapol. 2010. "The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production." Int. J. Mol. Sci. 11, no. 5: 2200-2211.