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Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)
Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Mahdasht Road, Karaj, Iran
Iranain National Gene Bank, Mahdasht Road, Karaj, Iran
* Author to whom correspondence should be addressed.
Received: 11 March 2010; in revised form: 17 April 2010 / Accepted: 27 April 2010 / Published: 3 May 2010
Abstract: Pomegranate (Punica granatum L.) has been cultivated from ancient times for its economic, ornamental and medicinal properties globally. Here, we report the isolation and characterization of 12 polymorphic microsatellite markers from a repeat-enriched genomic library of Punica granatum L. The genetic diversity of these loci was assessed in 60 genotypes of Punica granatum L. All loci were variable: the number of polymorphic alleles per locus ranged from two to five (average 2.9). The observed and expected heterozygosities ranged from 0.15 to 0.87 and 0.29 to 0.65, respectively. The polymorphic information content ranged from 0.26 to 0.61 (average: 0.43). To the best of our knowledge, this is the first time that polymorphic microsatellite markers have been reported for P. granatum L. These new markers should allow studies of the population structure and genetic diversity of pomegranate to be performed in the future.
Keywords: Punica granatum; microsatellite; pomegranate; SSR
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Pirseyedi, S.M.; Valizadehghan, S.; Mardi, M.; Ghaffari, M.R.; Mahmoodi, P.; Zahravi, M.; Zeinalabedini, M.; Nekoui, S.M.K. Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.). Int. J. Mol. Sci. 2010, 11, 2010-2016.
Pirseyedi SM, Valizadehghan S, Mardi M, Ghaffari MR, Mahmoodi P, Zahravi M, Zeinalabedini M, Nekoui SMK. Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.). International Journal of Molecular Sciences. 2010; 11(5):2010-2016.
Pirseyedi, Seyed Mostafa; Valizadehghan, Sahar; Mardi, Mohsen; Ghaffari, Mohammad Reza; Mahmoodi, Parvaneh; Zahravi, Mehdi; Zeinalabedini, Mehrshad; Nekoui, Seyed Mojtaba Khayam. 2010. "Isolation and Characterization of Novel Microsatellite Markers in Pomegranate (Punica granatum L.)." Int. J. Mol. Sci. 11, no. 5: 2010-2016.