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Int. J. Mol. Sci. 2010, 11(2), 731-744; doi:10.3390/ijms11020731
Article

Cytotoxic Effects of 2-Bromopropane on Embryonic Development in Mouse Blastocysts

Received: 2 January 2010; Accepted: 10 February 2010 / Published: 11 February 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: 2-Bromopropane (2-BP), an alternative to ozone-depleting solvents, is used as a cleaning solvent. Here, we examined the cytotoxic effects of 2-bromopropane (2-BP) on mouse embryos at the blastocyst stage, subsequent embryonic attachment and outgrowth in vitro, and in vivo implantation via embryo transfer. Mouse blastocysts were incubated in medium with or without 2-BP (2.5, 5 or 10 μM) for 24 h. Cell proliferation and growth were investigated with dual differential staining, apoptosis was analyzed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) analysis, and implantation and post-implantation development of embryos were assessed using in vitro development analysis and in vivo embryo transfer, respectively. Blastocysts treated with 5 or 10 μM 2-BP displayed significantly increased apoptosis, and decreased inner cell mass (ICM) and trophectoderm (TE) cell number. Additionally, the implantation success rates of 2-BP-pretreated blastocysts were lower than those of untreated controls. In vitro treatment with 5 or 10 μM 2-BP was associated with increased resorption of postimplantation embryos, and decreased placental and fetal weights. Our results collectively indicate that in vitro exposure to 2-BP induces apoptosis, suppresses implantation rates after transfer to host mice, and retards early postimplantation development.
Keywords: 2-bromopropane; blastocyst; apoptosis; embryonic development 2-bromopropane; blastocyst; apoptosis; embryonic development
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chan, W.-H. Cytotoxic Effects of 2-Bromopropane on Embryonic Development in Mouse Blastocysts. Int. J. Mol. Sci. 2010, 11, 731-744.

AMA Style

Chan W-H. Cytotoxic Effects of 2-Bromopropane on Embryonic Development in Mouse Blastocysts. International Journal of Molecular Sciences. 2010; 11(2):731-744.

Chicago/Turabian Style

Chan, Wen-Hsiung. 2010. "Cytotoxic Effects of 2-Bromopropane on Embryonic Development in Mouse Blastocysts." Int. J. Mol. Sci. 11, no. 2: 731-744.


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